Toll樣受體4(TLR4)多克隆抗體
Polyclonal Antibody to Toll Like Receptor 4 (TLR4)
CD284; TOLL; HToll
- 編號(hào)PAA753Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來(lái)源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原RPA753Hu01-Toll樣受體4(TLR4)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.21mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; FCM
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格20μl100μl200μl1ml10ml
- 價(jià)格¥ 569¥ 1327¥ 1896¥ 4740¥ 18960
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Rat Liver lysate; Lane3: Rat Testis lysate Primary Ab: 1μg/ml Rabbit Anti-Human TLR4 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Human SH-SY5Y cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human TLR4 Polyclonal Antibody (Catalog PAA753Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)TLR4的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別TLR4。
用法
Western blotting: 0.01-2μg/mL;
Flow cytometry:20μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA753Hu01 | Toll樣受體4(TLR4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA753Hu01 | Toll樣受體4(TLR4)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA753Hu02 | Toll樣受體4(TLR4)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA753Hu02 | Toll樣受體4(TLR4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA753Hu03 | Toll樣受體4(TLR4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA753Hu04 | Toll樣受體4(TLR4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA753Hu21 | Toll樣受體4(TLR4)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA753Hu02 | Toll樣受體4(TLR4)多克隆抗體 | IHC |
| PAA753Hu01 | Toll樣受體4(TLR4)多克隆抗體 | WB; FCM |
| PAA753Hu08 | Toll樣受體4(TLR4)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA753Hu71 | Toll樣受體4(TLR4)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA753Hu25 | Toll樣受體4(TLR4)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA753Hu24 | Toll樣受體4(TLR4)單克隆抗體 | WB |
| MAA753Hu23 | Toll樣受體4(TLR4)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA753Hu21 | Toll樣受體4(TLR4)單克隆抗體 | WB |
| MAA753Hu22 | Toll樣受體4(TLR4)單克隆抗體 | WB |
| LAA753Hu72 | Toll樣受體4(TLR4)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MEA753Hu | Toll樣受體4(TLR4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA753Hu | Toll樣受體4(TLR4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA753Hu | Toll樣受體4(TLR4)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA753Hu01 | Toll樣受體4(TLR4)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA753Hu01 | Toll樣受體4(TLR4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Journal For?Neurovirology | Soluble?TLR2?and?4?concentrations?in?cerebrospinal?fluid?in?HIV/SIV-relatedneuropathological?conditions.[pubmed:27882497] |
| Acta?Orthopaedica | Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic joints[pubmed:28093922] |
| Environmental Toxicology and Pharmacology | Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro study[pubmed:28192751] |
| Inflammation | Toll-Like Receptor 4 Inhibition Improves Oxidative Stress and Mitochondrial Health in Isoproterenol-Induced Cardiac Hypertrophy in Rats[pubmed:28690610] |
| Comparative Immunology, Microbiology and Infectious Diseases | Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts[DOI: 10.1016/j.cimid.2017.06.002] |
| Comparative Immunology, Microbiology and Infectious Diseases | Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts[10.1016/j.cimid.2017.06.002] |
| Microbial Pathogenesis | Protective effects of sinomenine against LPS-induced inflammation in piglets.[pubmed:28757275] |
| Cytokine | Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects[pubmed:29102164] |
| Biomedicine & pharmacotherapy | Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms[pubmed:28061403] |
| Frontiers in Immunology | The soluble Form of Toll-like receptor 2 is elevated in serum of Multiple sclerosis Patients: a novel Potential Disease Biomarker[Pubmed:29593720] |
| Experimental Gerontology | Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia[Pubmed:30071285] |
| Neurotoxicity Research | Protective Effects of Fibroblast Growth Factor 21 Against Amyloid-Beta-Induced Toxicity in SH-SY5Y Cells[Pubmed:29869772] |
| European?Journal of Pharmacology | Rutin, a quercetin glycoside, alleviates acute endotoxemic kidney injury in C57BL/6 mice via suppression of inflammation and up-regulation of antioxidants and SIRT1[Pubmed:29920283] |
| Environmental Toxicology and Pharmacology | Crocin reverses unilateral renal ischemia reperfusion injury-induced augmentation of oxidative stress and toll like receptor-4 activity[Pubmed:29625388] |
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| Experimental?and?Therapeutic?Medicine | The HMGB1?RAGE/TLR?TNF?α signaling pathway may contribute to kidney injury induced by hypoxia[] |
| Immunopharmacology?and?immunotoxicology | Protective effect of sesamin in lipopolysaccharide-induced mouse model of acute kidney injury via attenuation of oxidative stress, inflammation, and apoptosis[Pubmed: 30488751] |
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| Biomedicine?& Pharmacotherapy | Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4[] |
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| Scientific Reports | Deregulation of TLR4 signaling pathway characterizes Bicuspid Aortic valve syndrome[Pubmed: 31363123] |
| Life Sciences | Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosis[Pubmed: 31465731] |
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| microbial pathogenesis | Protective effect of diosgenin on LPS/D-Gal-induced acute liver failure in C57BL/6 mice[Pubmed: 32389705] |
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| Putative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial?…[] | |
| The role of Toll-like receptors-4 in the pathogenesis of development of anemia of inflammation in young children[] | |
| Clin Oral Investig | Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces[34091792] |
| Eksp Klin Gastroenterol | Tissue transglutaminase is involved in the inflammatory processes of active chronic gastritis[17539352] |
| Iranian Journal of Medical Sciences | Esculetin Alleviates Acute Liver Failure following Lipopolysaccharide/D-Galactosamine in Male C57BL/6 Mice[] |
| Acta Obstet Gynecol Scand | A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis[34244993] |
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| Biomolecules | Vascular Calcification and the Gut and Blood Microbiome in Chronic Kidney Disease Patients on Peritoneal Dialysis: A Pilot Study[] |