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細(xì)胞色素P450家族成員2E1(CYP2E1)多克隆抗體

Polyclonal Antibody to Cytochrome P450 2E1 (CYP2E1)

CPE1; CYPIIE1; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; Cytochrome P450 Family 2 Subfamily E 1; Cytochrome P450,Subfamily IIE(Ethanol-Inducible)Polypeptide 1

編號(hào)PAA988Mu02
物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
來(lái)源多抗制備
宿主兔
效價(jià)-
Ig類型IgG
純化方式抗原特異性親和純化
標(biāo)記物無(wú)標(biāo)記物
免疫原RPA988Mu02-細(xì)胞色素P450家族成員2E1(CYP2E1)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度500μg/mL
且適物種Rattus norvegicus (Rat，大鼠)
應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。
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規(guī)格20μl100μl200μl1ml10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Samples: Mouse Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse CYP2E1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
FITC staining on IF;<br/>Samples: Human HepG2 cell; <br/>Primary Ab: 20μg/ml Rabbit Anti-Mouse CYP2E1 Antibody<br/>Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb18)

Figure. Western Blot; Sample: Recombinant CYP2E1, Mouse.
Western Blot; Sample: Mouse Liver lysate;
Primary Ab: 1μg/ml Rabbit Anti-Mouse CYP2E1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Liver lysate;
Primary Ab: 1μg/ml Rabbit Anti-Mouse CYP2E1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Mouse CYP2E1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Mouse CYP2E1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)CYP2E1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別CYP2E1。

用法

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽(yáng)性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長(zhǎng)臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
BMC Complementary and Alternative Medicine	Hepatoprotective effect of ethanolic extract of Curcuma longa on thioacetamide induced liver cirrhosis in rats[PubMed: PMC3605171]
Evidence-Based Complementary Alternative Medicine	Mechanism of Hepatoprotective Effect of Boesenbergia rotunda in Thioacetamide-Induced Liver Damage in Rats[PubMed: PMC3749608]
BMC Complementary and Alternative Medicine	CCl4-induced hepatotoxicity: protective effect of rutin on p53, CYP2E1 and the antioxidative status in rat[PubMed: PMC3519517]
Asian Journal of Biochemical and Pharmaceutical Research	Hepatoprotective Effect of Methanolic Stem Extract of Bush Cane (Costus Afer) on Immunologic Response Generated Reactive Oxygen Species (ROS) in Alcohol Induced Liver Cirrhosis in Rats. [Ajbpr:Source]
The Scientific World Journal	Pass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats[Pubmed:24701154]
Journal of Functional Foods	Protective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivo[publication:307092820]
CIENC TEC VITIVINIC,CIENCIA E TECNICA VITIVINICOLA	Haloperidol loaded carboxymethyl chitosan nanoparticles reduce Oxidative stress, Mitochondria dysfunction and DNA damage in hepatic cells of male rats.[59f7c85aaca272607e2d8d7a]
Environment International	Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures[Pubmed: 31639605]
Nutrients	Oxidative Stress Parameters in the Liver of Growing Male Rats Receiving Various Alcoholic Beverages[Pubmed: 31935882]
Antioxidants	Ellagic Acid Prevents Binge Alcohol-Induced Leaky Gut and Liver Injury through Inhibiting Gut Dysbiosis and Oxidative Stress[34573017]

編號(hào)	適用物種：Mus musculus (Mouse，小鼠)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA988Mu02	細(xì)胞色素P450家族成員2E1(CYP2E1)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPA988Mu01	細(xì)胞色素P450家族成員2E1(CYP2E1)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
APA988Mu01	細(xì)胞色素P450家族成員2E1(CYP2E1)活性蛋白	Cell?culture;?Activity?Assays.
APA988Mu02	細(xì)胞色素P450家族成員2E1(CYP2E1)活性蛋白	Cell?culture;?Activity?Assays.
PAA988Mu02	細(xì)胞色素P450家族成員2E1(CYP2E1)多克隆抗體	WB; IHC; ICC; IP.
PAA988Mu01	細(xì)胞色素P450家族成員2E1(CYP2E1)多克隆抗體	WB; IHC; ICC; IP.
LAA988Mu71	細(xì)胞色素P450家族成員2E1(CYP2E1)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
SEA988Mu	細(xì)胞色素P450家族成員2E1(CYP2E1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA988Mu	細(xì)胞色素P450家族成員2E1(CYP2E1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
KSA988Mu01	細(xì)胞色素P450家族成員2E1(CYP2E1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".

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