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細(xì)胞色素P450家族成員2E1(CYP2E1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Cytochrome P450 2E1 (CYP2E1)

CPE1; CYPIIE1; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; Cytochrome P450 Family 2 Subfamily E 1; Cytochrome P450,Subfamily IIE(Ethanol-Inducible)Polypeptide 1

編號(hào)KSA988Mu01
物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
試劑內(nèi)容包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
可檢測(cè)樣本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
可檢測(cè)范圍3.12-200ng/mL
可適靈敏度1.18ng/mL
應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
規(guī)格96T*596T*1096T*2096T*5096T*100
價(jià)格¥ 699811664¥ 11664¥ 20995¥ 40824¥ 69984
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)細(xì)胞色素P450家族成員2E1(CYP2E1)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。

配套試劑

贈(zèng)品

ELISA/CLIA實(shí)驗(yàn)服務(wù)

增值服務(wù)

化學(xué)發(fā)光試劑盒定制服務(wù)
酶聯(lián)免疫試劑盒定制服務(wù)
檢測(cè)試劑盒DIY輔助試劑包1
檢測(cè)試劑盒DIY輔助試劑包2
標(biāo)準(zhǔn)品稀釋液
 包被緩沖液
 抗體稀釋緩沖液
 檢測(cè)試劑盒DIY輔助試劑包3
檢測(cè)試劑盒DIY輔助試劑包4
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

相關(guān)產(chǎn)品

編號(hào)	適用物種：Mus musculus (Mouse，小鼠)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA988Mu02	細(xì)胞色素P450家族成員2E1(CYP2E1)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPA988Mu01	細(xì)胞色素P450家族成員2E1(CYP2E1)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
APA988Mu01	細(xì)胞色素P450家族成員2E1(CYP2E1)活性蛋白	Cell?culture;?Activity?Assays.
APA988Mu02	細(xì)胞色素P450家族成員2E1(CYP2E1)活性蛋白	Cell?culture;?Activity?Assays.
PAA988Mu02	細(xì)胞色素P450家族成員2E1(CYP2E1)多克隆抗體	WB; IHC; ICC; IP.
PAA988Mu01	細(xì)胞色素P450家族成員2E1(CYP2E1)多克隆抗體	WB; IHC; ICC; IP.
LAA988Mu71	細(xì)胞色素P450家族成員2E1(CYP2E1)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
SEA988Mu	細(xì)胞色素P450家族成員2E1(CYP2E1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA988Mu	細(xì)胞色素P450家族成員2E1(CYP2E1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
KSA988Mu01	細(xì)胞色素P450家族成員2E1(CYP2E1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志	參考文獻(xiàn)
BMC Complementary and Alternative Medicine	Hepatoprotective effect of ethanolic extract of Curcuma longa on thioacetamide induced liver cirrhosis in rats[PubMed: PMC3605171]
Evidence-Based Complementary Alternative Medicine	Mechanism of Hepatoprotective Effect of Boesenbergia rotunda in Thioacetamide-Induced Liver Damage in Rats[PubMed: PMC3749608]
BMC Complementary and Alternative Medicine	CCl4-induced hepatotoxicity: protective effect of rutin on p53, CYP2E1 and the antioxidative status in rat[PubMed: PMC3519517]
Asian Journal of Biochemical and Pharmaceutical Research	Hepatoprotective Effect of Methanolic Stem Extract of Bush Cane (Costus Afer) on Immunologic Response Generated Reactive Oxygen Species (ROS) in Alcohol Induced Liver Cirrhosis in Rats. [Ajbpr:Source]
The Scientific World Journal	Pass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats[Pubmed:24701154]
Journal of Functional Foods	Protective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivo[publication:307092820]
CIENC TEC VITIVINIC,CIENCIA E TECNICA VITIVINICOLA	Haloperidol loaded carboxymethyl chitosan nanoparticles reduce Oxidative stress, Mitochondria dysfunction and DNA damage in hepatic cells of male rats.[59f7c85aaca272607e2d8d7a]
Environment International	Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures[Pubmed: 31639605]
Nutrients	Oxidative Stress Parameters in the Liver of Growing Male Rats Receiving Various Alcoholic Beverages[Pubmed: 31935882]
Antioxidants	Ellagic Acid Prevents Binge Alcohol-Induced Leaky Gut and Liver Injury through Inhibiting Gut Dysbiosis and Oxidative Stress[34573017]