精氨酸酶(ARG)活性蛋白
Active Arginase (ARG)
ARG1; Arginase I; Liver Arginase
產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Arginase (ARG) is a kind of inducible manganese (Mn2 )-containing enzymes that catalyse?the?final?step?of?the?urea?cycle?in?the?liver,?converting?l?-arginine?into?l?-ornithine and urea.Therefore, we can measure its bioactivity by enzyme activity assay. Recombinant human Arg was diluted to different concentrations in deionized water.Loading 40 μL of the rhARG1 and 10ul Substrate mixture(100 mM Arginine, 500 mM Glycine, 1.25?mM?MnCl2, pH 10.5 ) into the plate. Incubated at 37 °C for 2 hours and stop the reaction with 200 μL stop solution of the 2 mM oPA, 2 mM NED ,50 mM Boric Acid, 1 M Sulfuric Acid, 0.03% Brij-35 (w/v) .Cover the plate and incubate at room temperature for 20 minutes and read at 520 nm (absorbance) in endpoint mode. The specific activity of recombinant human ARG is >6000pmol/min/ug.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APB120Hu02 | 精氨酸酶(ARG)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB120Hu01 | 精氨酸酶(ARG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB120Hu01 | 精氨酸酶(ARG)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB120Hu01 | 精氨酸酶(ARG)多克隆抗體 | WB; IHC |
| PAB120Hu02 | 精氨酸酶(ARG)多克隆抗體 | WB |
| LAB120Hu71 | 精氨酸酶(ARG)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAB120Hu81 | 精氨酸酶(ARG)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAB120Hu22 | 精氨酸酶(ARG)單克隆抗體 | WB; IHC |
| MAB120Hu23 | 精氨酸酶(ARG)單克隆抗體 | WB; IHC; ICC/IF |
| MAB120Hu24 | 精氨酸酶(ARG)單克隆抗體 | WB; IHC |
| MAB120Hu21 | 精氨酸酶(ARG)單克隆抗體 | WB; IHC |
| LAB120Hu72 | 精氨酸酶(ARG)單克隆抗體(生物素標記) | IHC |
| SEB120Hu | 精氨酸酶(ARG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB120Hu | 精氨酸酶(ARG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB120Hu01 | 精氨酸酶(ARG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| PLoS One | Phenobarbital Induces Alterations in the Proteome of Hepatocytes and Mesenchymal Cells of Rat Livers[Pubmed: 24204595] |
| J Cereb Blood Flow Metab | Arginase I release from activated neutrophils induces peripheral immunosuppression in a murine model of stroke[PubMed: 25966956] |
| Cancer Medicine | Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease[Pubmed:27109254] |
| Cell Mol Immunol? | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica[Pubmed:27157496] |
| Potent but transient immunosuppression of T-cells is a general feature of erythroid progenitor cells[] | |
| Commun Biol | Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells[34893694] |