精氨酸酶(ARG)活性蛋白
Active Arginase (ARG)
ARG1; Arginase I; Liver Arginase
- 編號(hào)APB120Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)6.7
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Gene sequencing
Figure. SDS-PAGE
WB圖
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Arginase (ARG) is a kind of inducible manganese (Mn2 )-containing enzymes that catalyse?the?final?step?of?the?urea?cycle?in?the?liver,?converting?l?-arginine?into?l?-ornithine and urea.Therefore, we can measure its bioactivity by enzyme activity assay. Recombinant human Arg was diluted to different concentrations in deionized water.Loading 40 μL of the rhARG1 and 10ul Substrate mixture(100 mM Arginine, 500 mM Glycine, 1.25?mM?MnCl2, pH 10.5 ) into the plate. Incubated at 37 °C for 2 hours and stop the reaction with 200 μL stop solution of the 2 mM oPA, 2 mM NED ,50 mM Boric Acid, 1 M Sulfuric Acid, 0.03% Brij-35 (w/v) .Cover the plate and incubate at room temperature for 20 minutes and read at 520 nm (absorbance) in endpoint mode. The specific activity of recombinant human ARG is >24400pmol/min/ug.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB120Hu01 | 精氨酸酶(ARG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB120Hu01 | 精氨酸酶(ARG)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB120Hu01 | 精氨酸酶(ARG)多克隆抗體 | WB,IHC |
| PAB120Hu02 | 精氨酸酶(ARG)多克隆抗體 | WB |
| LAB120Hu71 | 精氨酸酶(ARG)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAB120Hu81 | 精氨酸酶(ARG)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAB120Hu22 | 精氨酸酶(ARG)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB120Hu23 | 精氨酸酶(ARG)單克隆抗體 | WB; IHC; ICC/IF |
| MAB120Hu24 | 精氨酸酶(ARG)單克隆抗體 | WB; IHC |
| MAB120Hu21 | 精氨酸酶(ARG)單克隆抗體 | WB; IHC |
| LAB120Hu72 | 精氨酸酶(ARG)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEB120Hu | 精氨酸酶(ARG)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB120Hu | 精氨酸酶(ARG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB120Hu01 | 精氨酸酶(ARG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS One | Phenobarbital Induces Alterations in the Proteome of Hepatocytes and Mesenchymal Cells of Rat Livers[Pubmed: 24204595] |
| J Cereb Blood Flow Metab | Arginase I release from activated neutrophils induces peripheral immunosuppression in a murine model of stroke[PubMed: 25966956] |
| Cancer Medicine | Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease[Pubmed:27109254] |
| Cell Mol Immunol? | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica[Pubmed:27157496] |
| Potent but transient immunosuppression of T-cells is a general feature of erythroid progenitor cells[] | |
| Commun Biol | Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells[34893694] |