細胞色素P450家族成員1A1(CYP1A1)重組蛋白
Recombinant Cytochrome P450 1A1 (CYP1A1)
CYP1; AHH; AHRR; CP11; CYP1; P1-450; P450-C; P450DX; Cytochrome P450,Subfamily I(Aromatic Compound-Inducible),Polypeptide 1
- 編號RPD295Ra01
- 物種Rattus norvegicus (Rat,大鼠)相同的名稱,不同的物種。
- 來源原核表達
- 宿主E.coli
- 內毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位線粒體, 內質網(wǎng)腔
- 預測分子量61.5kDa
- 實際分子量62kDa(差異分析請參閱說明書)
- 片段與標簽Ser251~His521 with N-terminal His and GST Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 95%
- 等電點6.8
- 應用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
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序列

用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當?shù)臈l件下存儲,損失率低于5%。
增值服務
相關產品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPD295Ra01 | 細胞色素P450家族成員1A1(CYP1A1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APD295Ra01 | 細胞色素P450家族成員1A1(CYP1A1)活性蛋白 | Cell?culture;?Activity?Assays. |
PAD295Ra01 | 細胞色素P450家族成員1A1(CYP1A1)多克隆抗體 | WB; IHC; ICC; IP. |
MAD295Ra21 | 細胞色素P450家族成員1A1(CYP1A1)單克隆抗體 | WB; IHC; ICC; IP. |
SED295Ra | 細胞色素P450家族成員1A1(CYP1A1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMD295Ra | 細胞色素P450家族成員1A1(CYP1A1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSD295Ra01 | 細胞色素P450家族成員1A1(CYP1A1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Journal of Nutrition | Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats[Pubmed:25733458] |
The Journal of Nutrition | Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,3[PubMed: 25733458] |
Molecular Nutrition Food Research | Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats[Pubmed:27133590] |
International Journal of Chemical and Natural Science | Caspase-3, Bcl-2, p53, CYP1A1 and COX -2 as a potential target in chemoprevention of Benzo (a) pyrene-induced lung carcinogenesis in mice: Role of thymoquinone[portal:uploads] |
International Journal of Pharma Sciences | Chemopreventive Role of Curcumin in benzo(A)pyrene induced Lung Carcinogenesis in mice via-modulation of Bcl-2, p53, Caspase-3, Cyp1A1, COX-2 and antioxidant defense system in Lung tissues[portal:uploads] |
Molecular Nutrition &?Food?Research | Phenethyl isothiocyanate?and?indole-3-carbinol?from?cruciferous?vegetables, but not furanocoumarins from apiaceous?vegetables, reduced PhIP-induced DNA adducts in Wistar rats.[pubmed:27133590] |
Biochemistry (Mosc) | Cytochrome P450 1A1 (CYP1A1) Catalyzes Lipid Peroxidation of Oleic Acid-Induced HepG2 Cells[Pubmed:29738693] |