表皮生長因子受體(EGFR)多克隆抗體
Polyclonal Antibody to Epidermal Growth Factor Receptor (EGFR)
ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
- 編號PAA757Hu02
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPA757Hu02-表皮生長因子受體(EGFR)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 565¥ 1319¥ 1884¥ 4710¥ 18840
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Human Lung cancer Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human EGFR Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Tonsil Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human EGFR Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human EGFR Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: Human Hela cell;
Primary Ab: 20ug/ml Rabbit Anti-Human EGFR Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 20ug/ml Rabbit Anti-Human EGFR Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
Western Blot; Sample: Lane1: A431 cell lysate; Lane2: Human Placenta lysate; Lane3: Porcine Cerebrum lysate Primary Ab: 0.1μg/ml Rabbit Anti-Human EGFR Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Human MCF7(3ul) cell lysate; <br/>Primary Ab: 1μg/ml Rabbit Anti-Human EGFR Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human EGFR Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對EGFR的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別EGFR。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA757Hu01 | 表皮生長因子受體(EGFR)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA757Hu02 | 表皮生長因子受體(EGFR)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA757Hu62 | 表皮生長因子受體(EGFR)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA757Hu01 | 表皮生長因子受體(EGFR)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA757Hu61 | 表皮生長因子受體(EGFR)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPA757Hu62 | 表皮生長因子受體(EGFR)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA757Hu02 | 表皮生長因子受體(EGFR)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA757Hu61 | 表皮生長因子受體(EGFR)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA757Hu06 | 表皮生長因子受體(EGFR)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA757Hu02 | 表皮生長因子受體(EGFR)多克隆抗體 | WB; IHC; ICC/IF |
| PAA757Hu01 | 表皮生長因子受體(EGFR)多克隆抗體 | WB; ICC/IF |
| FAA757Hu51 | 抗表皮生長因子受體(EGFR)多克隆抗體(別藻藍蛋白標記) | FCM |
| MAA757Hu27 | 表皮生長因子受體(EGFR)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA757Hu29 | 表皮生長因子受體(EGFR)單克隆抗體 | WB; IHC; ICC; IP. |
| RAA757Hu21 | 表皮生長因子受體(EGFR)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAA757Hu21 | 表皮生長因子受體(EGFR)單克隆抗體 | WB; IHC |
| MAA757Hu23 | 表皮生長因子受體(EGFR)單克隆抗體 | WB; ICC/IF |
| MAA757Hu24 | 表皮生長因子受體(EGFR)單克隆抗體 | WB; IHC; ICC/IF |
| MAA757Hu25 | 表皮生長因子受體(EGFR)單克隆抗體 | WB; ICC/IF |
| MAA757Hu22 | 表皮生長因子受體(EGFR)單克隆抗體 | WB; IHC |
| SEA757Hu | 表皮生長因子受體(EGFR)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA757Hu | 表皮生長因子受體(EGFR)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA757Hu | 抗表皮生長因子受體抗體(Anti-EGFR)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMA757Hu | 表皮生長因子受體(EGFR)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Thoracic Cancer | Nimotuzumab combined with gemcitabine and cisplatin as second-line chemotherapy for advanced non-small-cell lung cancer[Wiley: source] |
| J Cent Nerv Syst Dis.? | Increased Epidermal Growth Factor Receptor (EGFR) Associated with Hepatocyte Growth Factor (HGF) and Symptom Severity in Children with Autism …[Pubmed:25249767] |
| Mol Cancer Ther | GC1118, an anti-EGFR antibody with a distinct binding epitope and superior inhibitory activity against high-affinity EGFR ligands[PubMed: 26586721] |
| Cell Death Dis.? | LINC01225 promotes occurrence and metastasis of hepatocellular carcinoma in an epidermal growth factor receptor-dependent pathway[Pubmed:26938303] |
| analytica chimica acta | Microfluidic immunosensor based on mesoporous silica platform and CMK-3/poly-acrylamide-co-methacrylate of dihydrolipoic acid modified gold electrode for cancer biomarker detection.[pubmed:28335979] |
| Talanta | EGFR detection in extracellular vesicles of breast cancer patients through immunosensor based on silica-chitosan nanoplatform[Doi: 10.1016/j.talanta.2018.10.016] |
| Bioorganic Chemistry | Design, synthesis, molecular docking and biological activity evaluation of some novel indole derivatives as potent anticancer active agents and apoptosis inducers[Pubmed: 30665034] |
| Journal of?enzyme?inhibition and medicinal chemistry | Novel thienopyrimidine derivatives as dual EGFR and VEGFR-2 inhibitors: design, synthesis, anticancer activity and effect on cell cycle profile[Pubmed: 30919701] |
| Bioorganic Chemistry | Design and synthesis of new benzoxazole/benzothiazole-phthalimide hybrids as antitumor-apoptotic agents[] |
| Bioorganic Chemistry | Synthesis and anticancer activity of bis-benzo [d][1, 3] dioxol-5-yl thiourea derivatives with molecular docking study[Pubmed: 31288134] |
| Oxidative Medicine and Cellular Longevity | Evaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters?…[Pubmed: 31737175] |
| Neoplasia | Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine[Pubmed: 33212364] |
| Association between Serum Epidermal Growth Factor Receptor and Cyclooxygenase-2 Levels in Patients with Non-small Cell Carcinoma of Lung[] | |
| Synthesis, EGFR-TK inhibition and anticancer activity of new quinoxaline derivatives[] | |
| IMMUNOLOGIC RESEARCH | Humoral immune response to epidermal growth factor receptor in lung cancer[33495907] |
| European Journal of Medicinal Chemistry | Pyrazolo[3,4-d]pyrimidine-based dual EGFR T790M/HER2 inhibitors: Design, synthesis, structure¨Cactivity relationship and biological activity as potential antitumor and anticonvulsant agents[33545637] |
| Journal of Molecular Structure | Design, synthesis, antiproliferative evaluation, and molecular docking study of new quinoxaline derivatives as apoptotic inducers and EGFR inhibitors[] |
| ACS Appl Mater Interfaces | Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions[34914377] |
| Bioorganic Chemistry | Discovery of new pyrimido [5, 4-c] quinolines as potential antiproliferative agents with multitarget actions: Rapid synthesis, docking, and ADME studies[Pubmed:35219045] |