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Toll樣受體4(TLR4)單克隆抗體

Monoclonal Antibody to Toll Like Receptor 4 (TLR4)

CD284; TOLL; HToll

編號(hào)MAA753Cp21
物種Capra hircus; Caprine (Goat，山羊)相同的名稱，不同的物種。
來源單抗制備
宿主小鼠
效價(jià)(克隆號(hào))C4
Ig亞型 IgG1 Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無標(biāo)記物
免疫原RPA753Cp01-Toll樣受體4(TLR4)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度1mg/mL
且適物種Homo sapiens (Human，人)
應(yīng)用WB
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。
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規(guī)格20μl100μl200μl1ml10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

Western Blot; Sample: Lane1: Caprine Testis lysate; Lane2: Hela cell lysate Primary Ab: 0.2μg/ml Mouse Anti-Caprine TLR4 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)TLR4的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別TLR4。

用法

Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長(zhǎng)臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Chinese Medical Journal	Expression of soluble Toll-like receptors in pleural effusions[CMJ: 2010823483999506297]
Journal of Maternal-Fetal and Neonatal Medicine	Amniotic fluid soluble Toll-like receptor 4 in pregnancies complicated by preterm prelabor rupture of the membranes[Informa: 14767058.2011.626821]
PLoS One	Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation[PubMed: PMC3639905]
Clin Res Hepatol Gastroenterol.	The association between precancerous gastric lesions and serum pepsinogens, serum gastrin, vascular endothelial growth factor, serum interleukin-1 Beta, serum toll-like receptor-4 levels and Helicobacter pylori Cag A status[Pubmed: 23137754]
Journal of Clinical Microbiology	Toll-Like Receptor 2 in Serum: a Potential Diagnostic Marker of Prosthetic Joint Infection?[Pubmed: 24478497]
Surgical Endoscopy	CO2 pneumoperitoneum increases secretory IgA levels in the gut compared with laparotomy in an experimental animal model.[Pubmed: 24442682]
Experimental and therapeutic medicine	Soluble toll-like receptor 4 reversed attenuating effect of Chinese herbal Xiao-Qing-Long-Tang on allergen induced nerve growth factor and thymic stromal lymphopoietin[Pubmed: 24223644]
Free Radical Biology and Medicine	Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis[Pubmed:24992835]
Respiratory Virology and Immunogenicity Advances in Experimental Medicine and Biology	Cytokines and Toll-Like Receptors in the Immune Response to Influenza Vaccination[Pubmed:25248345]
Molecular and Cellular Biochemistry	Beta-arrestin-2 negatively modulates inflammation response in mouse chondrocytes induced by 4-mer hyaluronan oligosaccharide[Pubmed:25318610]
Journal of Ginseng Research	Effects of Korean Red Ginseng (Panax ginseng), urushiol (Rhus vernicifera Stokes), and probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus acidophilus R0052) on the gut–liver axis of alcoholic liver disease[Pubmed:25378990]
BMC Immunology	The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases[Pubmed:25406630]
Gastroenterology Research and Practice	Procoagulatory State in Inflammatory Bowel Diseases Is Promoted by Impaired Intestinal Barrier Function[Pubmed:25767508]
Eur J Gastroenterol Hepatol	Efficacy of rifaximin on circulating endotoxins and cytokines in patients with nonalcoholic fatty liver disease[PubMed: 26043290]
Pathol Biol (Paris)	Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in mice[PubMed: 25834936]
Journal of Food Science?	Effects of Hyriopsis cumingii Polysaccharides on Mice Immunologic Receptor, Transcription Factor, and Cytokine[Pubmed:27061742]
Plos One	Soluble?Toll-Like Receptors?2?and?4?in Cerebrospinal Fluid?of?Patients?with?Acute?Hydrocephalus?followingAneurysmal Subarachnoid Haemorrhage.[Pubmed:27223696]
Open Forum Infect Dis	The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-Patients[Pubmed:26835476]
Animal Feed Science and Technology	Impact of Saccharomyces cerevisiae fermentation product and subacute ruminal acidosis on production, inflammation, and fermentation in the rumen and hindCavia (Guinea pig )t of dairy cows[science:S0377840115300493]
european journal of pharmacology	Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms.[pubmed:27915041]
european journal of pharmacology	S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase.[pubmed:27887948]
Psychopharmacology (Berl)	Ellagic acid ameliorates learning and memory deficits in a rat model of Alzheimer's disease: an exploration of underlying mechanisms.[pubmed:28303372]
ЭНИ Забайкальский медицинский вестник	НОСИТЕЛЬСТВО ПОЛИМОРФИЗМА Thr399Ile и Asp299Gly ГЕНА TLR4 И УРОВЕНЬ TLR4 В ОПУХОЛЕВОЙ ТКАНИ У БОЛЬНЫХ ПРОЛИФЕРАТИВНЫМИ ЗАБОЛЕВАНИЯМИ МОЛОЧНОЙ ЖЕЛЕЗЫ[8.pdf]
biomedicine & pharmacotherapy	Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat.[pubmed:28199883]
Journal For?Neurovirology	Soluble?TLR2?and?4?concentrations?in?cerebrospinal?fluid?in?HIV/SIV-relatedneuropathological?conditions.[pubmed:27882497]
Acta?Orthopaedica	Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic joints[pubmed:28093922]
Environmental Toxicology and Pharmacology	Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro study[pubmed:28192751]
Inflammation	Toll-Like Receptor 4 Inhibition Improves Oxidative Stress and Mitochondrial Health in Isoproterenol-Induced Cardiac Hypertrophy in Rats[pubmed:28690610]
Comparative Immunology, Microbiology and Infectious Diseases	Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts[DOI: 10.1016/j.cimid.2017.06.002]
Comparative Immunology, Microbiology and Infectious Diseases	Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts[10.1016/j.cimid.2017.06.002]
Microbial Pathogenesis	Protective effects of sinomenine against LPS-induced inflammation in piglets.[pubmed:28757275]
Cytokine	Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects[pubmed:29102164]
Biomedicine & pharmacotherapy	Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms[pubmed:28061403]
Frontiers in Immunology	The soluble Form of Toll-like receptor 2 is elevated in serum of Multiple sclerosis Patients: a novel Potential Disease Biomarker[Pubmed:29593720]
Experimental Gerontology	Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia[Pubmed:30071285]
Neurotoxicity Research	Protective Effects of Fibroblast Growth Factor 21 Against Amyloid-Beta-Induced Toxicity in SH-SY5Y Cells[Pubmed:29869772]
European?Journal of Pharmacology	Rutin, a quercetin glycoside, alleviates acute endotoxemic kidney injury in C57BL/6 mice via suppression of inflammation and up-regulation of antioxidants and SIRT1[Pubmed:29920283]
Environmental Toxicology and Pharmacology	Crocin reverses unilateral renal ischemia reperfusion injury-induced augmentation of oxidative stress and toll like receptor-4 activity[Pubmed:29625388]
Physiology &?Behavior?	Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll?…[Pubmed:29410018]
International Journal of Gynecology & Obstetrics	Increased serum levels of high mobility group protein B1 and calprotectin in pre‐eclampsia[Pubmed:29569400]
Journal of Maternal-Fetal?&?Neonatal?Medicine	Increased TLR4 and TREM-1 expression on monocytes and neutrophils in preterm birth: further evidence of a proinflammatory state[Pubmed:29534643]
	ТLR4-Р?ВН? У ХВОРИХ НА ПОДАГРУ: ЗВ'ЯЗОК З АКТИВН?СТЮ ЗАХВОРЮВАННЯ[:]
Journal of?Medical?Virology	Combined effect of anti‐high‐mobility group box‐1 monoclonal antibody and peramivir against influenza A virus‐induced pneumonia in mice[Pubmed: 30281823]
Metabolic?Brain?Disease	Berberine ameliorates lipopolysaccharide-induced learning and memory deficit in the rat: insights into underlying molecular mechanisms[Pubmed: 30456649]
Experimental?and?Therapeutic?Medicine	The HMGB1?RAGE/TLR?TNF?α signaling pathway may contribute to kidney injury induced by hypoxia[]
Immunopharmacology?and?immunotoxicology	Protective effect of sesamin in lipopolysaccharide-induced mouse model of acute kidney injury via attenuation of oxidative stress, inflammation, and apoptosis[Pubmed: 30488751]
International Immunopharmacology	S-allyl cysteine protects against lipopolysaccharide-induced acute kidney injury in the C57BL/6 mouse strain: Involvement of oxidative stress and inflammation[Pubmed: 30665040]
Biomedicine?& Pharmacotherapy	Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4[]
International Journal of Obesity	Circulating microbiota-derived metabolites: a “l(fā)iquid biopsy?[Pubmed: 31388096]
Scientific Reports	Deregulation of TLR4 signaling pathway characterizes Bicuspid Aortic valve syndrome[Pubmed: 31363123]
Life Sciences	Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosis[Pubmed: 31465731]
Respiratory physiology & neurobiology	Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosis[Pubmed: 31600583]
Thrombosis and haemostasis	Particulate Shiga Toxin 2 in Blood is Associated to the Development of Hemolytic Uremic Syndrome in Children[Pubmed: 31858520]
J Ethnopharmacol	Effects of fermented ginseng on the gut microbiota and immunity of rats with antibiotic-associated diarrhea[Pubmed: 33217518]
International Journal of Molecular Sciences	Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis[Pubmed: 32545403]
NEUROPSYCHOBIOLOGY	Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying Mechanisms[Pubmed: 32526752]
journal of biochemical and molecular toxicology	S‐allyl cysteine, an active ingredient of garlic, attenuates acute liver dysfunction induced by lipopolysaccharide/ d‐galactosamine in mouse: Underlying mechanisms[Pubmed: 32453893]
microbial pathogenesis	Protective effect of diosgenin on LPS/D-Gal-induced acute liver failure in C57BL/6 mice[Pubmed: 32389705]
Sciences of Europe	FEATURES OF MYOCARDIAL REMODELING IN MEN WITH POST-INFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH TOLL-LIKE …[]
	Putative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial?…[]
	The role of Toll-like receptors-4 in the pathogenesis of development of anemia of inflammation in young children[]
Clin Oral Investig	Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces[34091792]
Eksp Klin Gastroenterol	Tissue transglutaminase is involved in the inflammatory processes of active chronic gastritis[17539352]
Iranian Journal of Medical Sciences	Esculetin Alleviates Acute Liver Failure following Lipopolysaccharide/D-Galactosamine in Male C57BL/6 Mice[]
Acta Obstet Gynecol Scand	A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis[34244993]
Nutrients	Expression of Jejunal Taste Receptors in Women with Morbid Obesity[34371946]
Biomed Pharmacother	Nifuroxazide-loaded cubosomes exhibit an advancement in pulmonary delivery and attenuate bleomycin-induced lung fibrosis by regulating the STAT3 and?…[Pubmed:35220029]
Biomed Pharmacother	Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an?…[Pubmed:35202914]
Frontiers in Pharmacology	Carbocisteine as a Modulator of Nrf2/HO-1 and NFκB Interplay in Rats: New Inspiration for the Revival of an Old Drug for Treating Ulcerative Colitis. Front?…[Pubmed:35754464]
Processes	Lipocalin, Resistin and Gut Microbiota-Derived Propionate Could Be Used to Predict Metabolic Bariatric Surgery Selected Outcomes[]
Biomolecules	Vascular Calcification and the Gut and Blood Microbiome in Chronic Kidney Disease Patients on Peritoneal Dialysis: A Pilot Study[]

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