角蛋白4(CK4)單克隆抗體
Monoclonal Antibody to Cytokeratin 4 (CK4)
CYK4; KRT4; K4; Keratin 4; Keratin, type II cytoskeletal 4; Type-II keratin Kb4
- 編號MAA489Ra21
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)1:270000 (1#)
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原RPA489Ra01-角蛋白4(CK4)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/mL
- 且適物種Homo sapiens (Human，人), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 652¥ 1520¥ 2172¥ 5430¥ 21720
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P;
Sample: Porcine Uterus Tissue
Primary Ab: 30μg/ml Mouse Anti-Rat CK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Porcine Liver Tissue
Primary Ab: 30μg/ml Mouse Anti-Rat CK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Porcine Lung Tissue
Primary Ab: 30μg/ml Mouse Anti-Rat CK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Porcine Pancreas Tissue
Primary Ab: 30μg/ml Mouse Anti-Rat CK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Figure.Western Blot;Sample: Recombinant KRT4, Rat.
Western Blot; Samples: Lane1: A431 cell lysate; Lane2: A549 cell lysate; Lane3: Hela cell lysate; Lane4: MCF7 cell lysate;
Primary Ab: 0.2μg/ml Mouse Anti-Rat CK4 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對CK4的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別CK4。
用法
Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA489Ra01 | 角蛋白4(CK4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA489Ra01 | 角蛋白4(CK4)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA489Ra71 | 角蛋白4(CK4)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA489Ra21 | 角蛋白4(CK4)單克隆抗體 | WB; IHC; ICC; IP. |
參考文獻
| 雜志 | 參考文獻 |
| Molecular?Medicine?Reports | Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranes[Pubmed:29749501] |
| FEBS Open Bio | Development of a novel murine heart failure model overexpressing human renin and angiotensinogen[Pubmed: 32056390] |
| J Clin Periodontol | Discovery, validation, and diagnostic ability of multiple protein-based biomarkers in saliva and gingival crevicular fluid to distinguish between health and periodontal?…[Pubmed:35451104] |