白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)
Mini Samples ELISA Kit for Interleukin 4 (IL4)
BSF1; BCGF1 ;B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra
- 編號MEA077Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于6.2pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
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產品包裝(模擬)
產品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 84-95 | 88 |
| EDTA plasma(n=5) | 98-105 | 102 |
| heparin plasma(n=5) | 80-99 | 91 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-103% | 91-98% | 95-105% | 82-102% |
| EDTA plasma(n=5) | 82-99% | 78-95% | 99-105% | 84-94% |
| heparin plasma(n=5) | 80-103% | 78-94% | 81-89% | 91-102% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）25µL，37°C溫育1小時；
3. 吸棄，加檢測溶液A25µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B25µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物25µL，37°C孵育10-20分鐘；
8. 加終止液20µL，立即450nm讀數。
實驗原理
將白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)與連接于固相載體上的抗體結合，然后加入生物素化的白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| EPA077Hu61 | 白介素4(IL4)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA077Hu61 | 白介素4(IL4)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA077Hu01 | 白介素4(IL4)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA077Hu01 | 白介素4(IL4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA077Hu02 | 白介素4(IL4)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA077Hu06 | 白介素4(IL4)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA077Hu01 | 白介素4(IL4)多克隆抗體 | IHC; ICC/IF |
| MAA077Hu21 | 白介素4(IL4)單克隆抗體 | IHC |
| MAA077Hu22 | 白介素4(IL4)單克隆抗體 | WB; IHC; ICC; IP. |
| RAA077Hu21 | 白介素4(IL4)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MEA077Hu | 白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA077Hu | 白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA077Hu | 白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA077Hu | 白介素4(IL4)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA077Hu | 白介素4(IL4)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA077Hu01 | 白介素4(IL4)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
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| Frontiers in Pharmacology | M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in Mice[Pubmed: 33041798] |
| Biomedicine & Pharmacotherapy | Qingfei oral liquid alleviates airway hyperresponsiveness and mucus hypersecretion via TRPV1 signaling in RSV-infected asthmatic mice[Pubmed: 32521453] |
| Journal of Traditional Chinese Medical Sciences | Mahuang Xixin Fuzi Decoction protects against ovalbumin-induced allergic rhinitis by inhibiting type 2 innate lymphoid cells in mice[] |
| 畜牧獸醫(yī)學報 | 雞骨髓源樹突狀細胞體外誘導[] |
| Evaluation of lipopolysaccharide-induced acute lung injury attenuation in mice by Glycyrrhiza glabra[] | |
| ACS Biomaterials Science & Engineering | Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction[] |
| Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction by enhancing Th2 cytokine[] | |
| Scientific Reports | Immunogenicity and protective efficacy of enterotoxigenic Escherichia coli (ETEC) total RNA against ETEC challenge in a mouse model[33239756] |
| Animal | Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves[33610512] |
| Biomed Pharmacother | Qingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic mice[33706133] |
| Life Sci | Asiatic acid attenuates diabetic retinopathy through TLR4/MyD88/NF-|êB p65 mediated modulation of microglia polarization[33965378] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies[33999928] |
| Brain Res Bull | Melatonin ameliorates anxiety-like behaviors induced by sleep deprivation in mice: role of oxidative stress, neuroinflammation, autophagy and apoptosis[34144202] |
| Vaccines | Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious??-[33670249] |
| cells | The Effect of the Clenbuterol?a|?2-Adrenergic Receptor Agonist on the Peripheral Blood Mononuclear Cells Proliferation, Phenotype, Functions, and Reactive Oxygen??-[33920705] |
| World J Tradit Chin Med | Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction via Th2 cytokine enhancement[] |
| Sci Rep | Talaromyces marneffei and nontuberculous mycobacteria co-infection in HIV-negative patients[34376749] |
| J Immunol Res | Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus[34395633] |
| Biomed Pharmacother | Allicin ameliorates renal ischemia/reperfusion injury via inhibition of oxidative stress and inflammation in rats[34426252] |
| J Periodontol | Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study[34730850] |
| Transl Psychiatry | Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive?…[34802039] |
| Inflamm Res | Dexmedetomidine exerts cerebral protective effects against cerebral ischemic injury by promoting the polarization of M2 microglia via the Nrf2/HO-1/NLRP3 pathway[34767031] |
| Animal Nutrition | Optimizing the growth and immune system of dairy calves by subdividing the pre-weaning period and providing different milk volumes for each stage[34786502] |
| J Inflamm Res | Clenbuterol, a Selective β2-Adrenergic Receptor Agonist, Inhibits or Limits Post-Stroke Pneumonia, but Increases Infarct Volume in MCAO Mice[Pubmed:35058704] |
| Mol Cell Biochem | The microRNA-1278/SHP-1/STAT3 pathway is involved in airway smooth muscle cell proliferation in a model of severe asthma both intracellularly and extracellularly[Pubmed:35157180] |
| Toxicology | Protection from Benzene-induced Immune Dysfunction in Mice[Pubmed:35090963] |
| Biomed Pharmacother | Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis[Pubmed:35453007] |
| Journal of Biomaterials Applications | Photobiomodulation associated with lipid nanoparticles and hyaluronic acid accelerate the healing of excisional wounds[Pubmed:35705485] |
| Viruses | Synergistic Pathogenicity by Coinfection and Sequential Infection with NADC30-like PRRSV and PCV2 in Post-Weaned Pigs[Pubmed:35215787] |
| researchsquare | Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling[] |
| Mljekarstvo | Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows[] |
