胱天蛋白酶3(CASP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)
Mini Samples ELISA Kit for Caspase 3 (CASP3)
CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1
- 編號MEA626Ra
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.055ng/mL.
- 樣本類型tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測胱天蛋白酶3(CASP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）25µL，37°C溫育1小時；
3. 吸棄，加檢測溶液A25µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B25µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物25µL，37°C孵育10-20分鐘；
8. 加終止液20µL，立即450nm讀數(shù)。
實驗原理
將胱天蛋白酶3(CASP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的胱天蛋白酶3(CASP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的胱天蛋白酶3(CASP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的胱天蛋白酶3(CASP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA626Ra02 | 胱天蛋白酶3(CASP3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA626Ra01 | 胱天蛋白酶3(CASP3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA626Ra03 | 胱天蛋白酶3(CASP3)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA626Ra04 | 胱天蛋白酶3(CASP3)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA626Ra02 | 胱天蛋白酶3(CASP3)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA626Ra03 | 胱天蛋白酶3(CASP3)多克隆抗體 | WB |
| PAA626Ra01 | 胱天蛋白酶3(CASP3)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA626Ra71 | 胱天蛋白酶3(CASP3)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA626Ra81 | 胱天蛋白酶3(CASP3)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA626Ra21 | 胱天蛋白酶3(CASP3)單克隆抗體 | WB; IHC; ICC; IP. |
| LAA626Ra82 | 胱天蛋白酶3(CASP3)單克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| HEA626Ra | 胱天蛋白酶3(CASP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA626Ra | 胱天蛋白酶3(CASP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA626Ra | 胱天蛋白酶3(CASP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA626Ra | 胱天蛋白酶3(CASP3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA626Ra01 | 胱天蛋白酶3(CASP3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
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| International Journal of Research in Pharmaceutical | In situ gel loaded with curcumin-based fluvastatin nanostructured carrier effective against squamous tongue carcinoma[] |
| SAGE Journals | Insights Into Protective Mechanisms of Dandelion Leaf Extract Against Cisplatin-Induced Nephrotoxicity in Rats: Role of Inhibitory Effect on Inflammatory and Apoptotic?…[] |
| Bangladesh Journal of Pharmacology | Ethylmethylhydroxypyridine succinate, acetylcysteine and choline alphoscerate improve mitochondrial function under condition of cerebral ischemia in rat[] |
| International Journal of Pharmacology | Ellagic Acid Loaded TPGS Micelles for Enhanced Anticancer Activities in Ovarian Cancer[] |
| Strawberry (Fragaria x ananassa) leaf extract reduces brain damage in diabetic male rats[] | |
| Indian Journal of Animal Research | Accacia senegel extract ameliorates Lead Acetate-mediated Hepatotoxicity in rats.[] |
| RESEARCH SQUARE | Experimental Observation of the Sequence of Tibial Plateau Chondrocyte and Matrix Degeneration in Spontaneous Osteoarthritis in Guinea Pigs[] |
| Egyptian Journal of Basic and Applied Sciences | Fish-oil supplementation improves retinal injury induced by diabetes and hypercholesterolemia in male Wistar rats[] |
| ACTIVITY OF APOPTOSIS MARKERS IN HPV-ASSOCIATED CERVICAL PATHOLOGIES[] | |
| Norwegian Journal of development of the International Science | CLINICAL AND PATHOGENETIC SIGNIFICANCE OF CHANGES IN PURINE METABOLISM DURING LUNG CANCER [] |
| J Biochem Mol Toxicol | Concanavalin A induces apoptosis in a dose?\dependent manner by modulating thiol/disulfide homeostasis in C6 glioblastoma cells[33604990] |
| International Journal of Reproductive Medicine | Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis[33575317] |
| Sci Rep | Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats[33750916] |
| TOXICOLOGY IN VITRO | Cyproheptadine causes apoptosis and decreases inflammation by disrupting thiol/disulfide balance and enhancing the levels of SIRT1 in C6 glioblastoma cells[33675893] |
| Biomed Pharmacother | Captopril downregulates expression of Bax/cytochrome C/caspase-3 apoptotic pathway, reduces inflammation, and oxidative stress in cisplatin-induced acute??-[33945910] |
| Reprod Sci | Reproductive Effects of Nicotinamide on Testicular Function and Structure in Old Male Rats: Oxidative, Apoptotic, Hormonal, and Morphological Analyses[34101148] |
| BMC Complement Med Ther | Effect of the standard herbal preparation, STW5, treatment on dysbiosis induced by dextran sodium sulfate in experimental colitis[34103031] |
| Environ Sci Pollut Res Int | Comparative effects of metformin and Cistus laurifolius L. extract in streptozotocin-induced diabetic rat model: oxidative, inflammatory, apoptotic, and histopathological??-[34097215] |
| Piceatannol Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by Modulation of Nrf2/HO-1/NF|êB Axis. Front. Pharmacol. 11: 614897. doi??-[33519479] | |
| Biomed Pharmacother | Allicin ameliorates renal ischemia/reperfusion injury via inhibition of oxidative stress and inflammation in rats[34426252] |
| Chem Biol Interact | Protective role of naringin loaded solid nanoparticles against aflatoxin B1 induced hepatocellular carcinoma[34717916] |
| Biochim Biophys Acta Gen Subj | Paraoxonase 2 protects against the CML mediated mitochondrial dysfunction through modulating JNK pathway in human retinal cells[34710487] |
| AAPS PharmSciTech | Development of an Icariin-Loaded Bilosome-Melittin Formulation with Improved Anticancer Activity against Cancerous Pancreatic Cells[34668089] |
| Analytical Cellular Pathology | The Synergistic Effect of TRPV1 on Oxidative Stress-Induced Autophagy and Apoptosis in Microglia[34336554] |
| Ecotoxicol Environ Saf | Evaluation of cytogenotoxic potential and embryotoxicity of KRS-Cauvery River water in zebrafish (Danio rerio)[Pubmed:35183813] |
| Environ Sci Pollut Res Int | Chrysin attenuates estradiol-induced endometrial hyperplasia in rats via enhancing PPARα activity[Pubmed:35301625] |
| PLoS One | Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats[Pubmed:35421180] |
| Journal of Ethnopharmacology | Ethnopharmacological impact of Melaleuca rugulosa (Link) Craven leaves extract on liver inflammation[Pubmed:35337921] |
| Eur J Pharmacol | Harmine prevents 3-nitropropionic acid-induced neurotoxicity in rats via enhancing NRF2-mediated signaling: Involvement of p21 and AMPK[Pubmed:35623405] |
| Gels | QbD Supported Optimization of the Alginate-Chitosan Nanoparticles of Simvastatin in Enhancing the Anti-Proliferative Activity against Tongue Carcinoma[Pubmed:35200484] |
| Naunyn-Schmiedeberg's Archives | Inflammatory Modulation of miR-155 Inhibits Doxorubicin?Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus?…[Pubmed:35715546] |
| Antioxidants | Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model?…[Pubmed:35204140] |
| Value Health Sci | Oksidatif, Apoptotik ve ?nflamatuar Sinyal Yolaklar? üzerinden C6 Glioblastoma Hücrelerindeki ML351'in Antiproliferatif Etkileri[] |
| Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation?…[] | |
| Biointerface Research in Applied Chemistry | Promising Therapeutic Efficacy of Trigonella-foenum graecum and Bone Marrow-Derived Mesenchymal Stem Cells on Skeletal Muscle Atrophy in Experimental?…[] |
| Arab Journal of Nuclear Sciences and Applications | Potential activity of silver/hydroxyapatite nano composite against damaged liver consequence to irradiation[] |
