S100鈣結合蛋白A12(S100A12)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for S100 Calcium Binding Protein A12 (S100A12) ,etc. by FLIA (Flow Luminescence Immunoassay)
CGRP; P6; CAAF1; CAGC; ENRAGE; MRP6; Calgranulin C; Calcium-binding protein in amniotic fluid 1; Extracellular newly identified RAGE-binding; Migration inhibitory factor-related 6
(注:單次混測多因子不超過8個指標 )
- 編號LMB080Hu
- 物種Homo sapiens (Human,人)相同的名稱,不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍4.88-5000pg/mL
- 靈敏度最小可檢測劑量小于等于1.627 pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格8指標數(shù)7指標數(shù)6指標數(shù)5指標數(shù)4指標數(shù)3指標數(shù)2指標數(shù)1指標數(shù)
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特異性
本試劑盒用于檢測S100鈣結合蛋白A12(S100A12)等多因子檢測試劑盒(流式熒光發(fā)光法),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的S100鈣結合蛋白A12(S100A12)等多因子檢測試劑盒(流式熒光發(fā)光法)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 95-102 | 99 |
EDTA plasma(n=5) | 93-105 | 101 |
heparin plasma(n=5) | 79-101 | 87 |
sodium citrate plasma(n=5) | 85-97 | 90 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內加入適量的S100鈣結合蛋白A12(S100A12)等多因子檢測試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中S100鈣結合蛋白A12(S100A12)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 82-97% | 80-102% | 82-91% | 91-104% |
EDTA plasma(n=5) | 99-105% | 89-96% | 81-96% | 88-97% |
heparin plasma(n=5) | 83-101% | 88-102% | 84-98% | 78-97% |
sodium citrate plasma(n=5) | 93-101% | 91-98% | 93-103% | 92-105% |
穩(wěn)定性
經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備;
2. 加樣(標準品、樣本、磁珠)標準品或樣本100μL及磁珠10μL,
37°C酶標板振蕩器孵育90分鐘;
3. 磁吸甩干,加檢測溶液A100μL,37°C酶標板振蕩器孵育60分鐘;
4. 磁吸洗板3次;
5. 加檢測溶液B100μL,37°C振動孵育30分鐘;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將S100鈣結合蛋白A12(S100A12)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標準品或標本以及磁珠,其中的S100鈣結合蛋白A12(S100A12)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合,然后加入生物素化的S100鈣結合蛋白A12(S100A12)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體,將未結合的生物素化抗體洗凈后,加入PE標記的親和素,再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的S100鈣結合蛋白A12(S100A12)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。
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參考文獻
雜志 | 參考文獻 |
Inflammation Research | Correlation of human S100A12 (EN-RAGE) and high-sensitivity C-reactive protein as gingival crevicular fluid and serum markers of inflammation in chronic periodontitis and type 2 diabetes[Pubmed: 24378957] |
Dig Dis Sci | The Circulating Level of Soluble Receptor for Advanced Glycation End Products Displays Different Patterns in Ulcerative Colitis and Crohn’s Disease: A Cross-Sectional Study[PubMed: 25757448] |
The Journal of Clinical Endocrinology & Metabolism | Low Endogenous Secretory Receptor for Advanced Glycation End-Products Levels Are Associated With Inflammation and Carotid Atherosclerosis in Prediabetes[Pubmed:26885882] |
Nutrition, Metabolism, and Cardiovascular Diseases | High intake of dietary advanced glycation end-products is associated with increased arterial stiffness and inflammation in subjects with type 2 diabetes.[pubmed:28958695] |
The Journal of Clinical Endocrinology & Metabolism | HbA1c Identifies Subjects With Prediabetes and Subclinical Left Ventricular Diastolic Dysfunction.[pubmed:28973588] |
Journal of Immunology Research | A Granulocyte-Specific Protein S100A12 as a Potential Prognostic Factor Affecting Aggressiveness of Therapy in Patients with Juvenile Idiopathic Arthritis[Doi: 10.1155/2018/5349837] |
Al-Azhar Assiut Medical Journal | The role of serum S100A12 (calgranulin C) as a diagnostic marker in Egyptian patients with irritable bowel syndrome and ulcerative colitis[Doi: 10.4103/AZMJ.AZMJ_52_18] |
Acta?Diabetologica | Analysis of S100A12 plasma levels in hyperlipidemic subjects with or without familial hypercholesterolemia[Pubmed: 30963307] |
Cells | 1 h Postload Glycemia Is Associated with Low Endogenous Secretory Receptor for Advanced Glycation End Product Levels and Early Markers of Cardiovascular?…[] |
Biomolecules . | Multimarker Approach to Identify Patients with Coronary Artery Disease at High Risk for Subsequent Cardiac Adverse Events: The Multi-Biomarker Study[Pubmed: 32549327] |
Mediators of Inflammation | Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris[] |
Animals (Basel) | The Serum and Saliva Proteome of Dogs with Diabetes Mellitus[33271797] |