誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Nitric Oxide Synthase 2, Inducible (NOS2) ,etc. by FLIA (Flow Luminescence Immunoassay)
NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2
（注：?jiǎn)未位鞙y(cè)多因子不超過(guò)8個(gè)指標(biāo) ）
- 編號(hào)LMA837Mu
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3.5h
- 檢測(cè)范圍0.02-20ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.007 ng/mL.
- 樣本類型tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格8指標(biāo)數(shù)7指標(biāo)數(shù)6指標(biāo)數(shù)5指標(biāo)數(shù)4指標(biāo)數(shù)3指標(biāo)數(shù)2指標(biāo)數(shù)1指標(biāo)數(shù)
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產(chǎn)品包裝(模擬)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測(cè)溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測(cè)溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈(zèng)品
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA837Mu03 | 誘導(dǎo)型一氧化氮合酶(NOS2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA837Mu01 | 誘導(dǎo)型一氧化氮合酶(NOS2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA837Mu04 | 誘導(dǎo)型一氧化氮合酶(NOS2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA837Mu01 | 誘導(dǎo)型一氧化氮合酶(NOS2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA837Mu02 | 誘導(dǎo)型一氧化氮合酶(NOS2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA837Mu01 | 誘導(dǎo)型一氧化氮合酶(NOS2)多克隆抗體 | WB; IHC |
| PAA837Mu02 | 誘導(dǎo)型一氧化氮合酶(NOS2)多克隆抗體 | WB |
| PAA837Mu03 | 誘導(dǎo)型一氧化氮合酶(NOS2)多克隆抗體 | WB; IHC |
| LAA837Mu71 | 誘導(dǎo)型一氧化氮合酶(NOS2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MEA837Mu | 誘導(dǎo)型一氧化氮合酶(NOS2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA837Mu | 誘導(dǎo)型一氧化氮合酶(NOS2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA837Mu | 誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA837Mu01 | 誘導(dǎo)型一氧化氮合酶(NOS2)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | High-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic mice[PubMed: 20713153] |
| Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | Hyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expression[ScienceDirect: S0925443911001323] |
| FEBS Journal | Adenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta[Wiley: source] |
| Gene | Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors[ScienceDirect: S0378111911007347] |
| PLoS One | A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric Oxide[PubMed: 23359814] |
| PLoS ONE | Regulation of Microglia Activity by Glaucocalyxin-A: Attenuation of Lipopolysaccharide-Stimulated Neuroinflammation through NF-κB and p38 MAPK Signaling Pathways[PlosOne: Source] |
| Journal of Cellular Biochemistry | The inhibition of hyaluronan degradation reduced pro-inflammatory cytokines in mouse synovial fibroblasts subjected to collagen-induced arthritis.[PubMed: 22234777] |
| International Immunopharmacology | Amentoflavone inhibits iNOS, COX-2 expression and modulates cytokine profile, NF-κB signal transduction pathways in rats with ulcerative colitis[Pubmed: 24126114] |
| Integrative Cancer Therapies | Decalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunits[Pubmed: 24013642] |
| Food and Chemical Toxicology | Inhibition of UVB-induced skin phototoxicity by a grape seed extract as modulator of nitrosative stress, ERK/NF-kB signaling pathway and apoptosis, in SKH-1 mice[Pubmed: 23567245] |
| Experimental Gerontology | Age-related changes in ADMA–DDAH–NO pathway in rat liver subjected to partial ischemia followed by global reperfusion[ScienceDirect: S0531556513003331] |
| International Immunopharmacology | Protective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunits[ScienceDirect: S1567576913004153] |
| INTERNATIONAL JOURNAL OF MEDICINE AND MEDICAL RESEARCH | L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION[Tdmu:Source] |
| Molecular Vision | Effects of topically applied tocotrienol on cataractogenesis and lens redox status in galactosemic rats[Pubmed:24940038] |
| Journal of Environmental Pathology, Toxicology and Oncology | Protective Effect of?Acacia ferruginea?against Ulcerative Colitis via Modulating Inflammatory Mediators, Cytokine Profile and NF-κB Signal Transduction Pathways[Pubmed:24941292] |
| Eur J Cancer Prev. | Utility of Apc-mutant rats with a colitis-associated colon carcinogenesis model for chemoprevention studies[Pubmed:25222193] |
| Cell and Tissue Research | Beta-arrestin 1 is involved in the catabolic response stimulated by hyaluronan degradation in mouse chondrocytes[Pubmed:25673209] |
| Ann N Y Acad Sci.? | Modulating effect of Biophytum sensitivum extract on rats with acetic acid-induced ulcerative colitis[Pubmed:5243883] |
| Molecular Medicine Reports | Resveratrol ameliorates oxidative stress and inhibits aquaporin 4 expression following rat cerebral ischemia-reperfusion injury[PubMed: 26458999] |
| Chem Biol Interact | Apocynin protects against ethanol-induced gastric ulcer in rats by attenuating the upregulation of NADPH oxidases 1 and 4[PubMed: 26522475] |
| Journal of Environmental Pathology, Toxicology and Oncology | Protective Effect of[PubMed: 26349607] |
| Journal of Immunotoxicology | Acacia ferruginea inhibits inflammation by regulating inflammatory iNOS and COX-2[PubMed: 25738525] |
| Cancer Medicine | Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease[Pubmed:27109254] |
| Journal of Natural Medicines | Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in mice[Pubmed:26972100] |
| Cell Mol Immunol? | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica[Pubmed:27157496] |
| Медицина и здравоохранение | Роль индуцибельной NO-синтазы в механизмах регуляции функциональной активности кальций-активируемых калиевых каналов коронарных сосудов у крыс, адаптированных к стрессу[article:n] |
| Graefe's Archive for Clinical and Experimental Ophthalmology | Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome[pubmed:27538909] |
| Taylor & Francis | Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation[09540105.2017.1306494] |
| Molecular Vision | Mechanism of the anticataract effect of liposomal MgT in galactose-fed rats.[pubmed:27440992] |
| Journal of Natural Medicines | Anti-oxidative,?anti-secretory?and?anti-inflammatory?activities?of the?extract?from the?root?bark?ofLycium?chinense?(Cortex Lycii) against gastric ulcer in mice.[pubmed:26972100] |
| Applied?Biochemistry?and?Biotechnology | Protective?Effect?of?Zingiber?officinale?Against?Dalton's?Lymphoma?Ascites?Tumour?byRegulating?Inflammatory?Mediator?and?Cytokines.[pubmed:27435276] |
| Cell Mol Neurobiol | Posttraumatic Stress Disorder Disturbs Coronary Tone and Its Regulatory Mechanisms[pubmed:28676988] |
| Microbial Immunology | Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota[pubmed:28713364] |
| Biomedicine & Pharmacotherapy | Protective effect of Averrhoa bilimbi L. fruit extract on ulcerative colitis in wistar rats via regulation of inflammatory mediators and cytokines[pubmed:28531922] |
| The Royal Society of Chemistry | Anti-ulcerogenic effect of KFP-H008 against ethanol-induced gastric ulcer via p38 MAPK/NF-κB pathway[10.1039/C7RA08879E] |
| Acta Pharmacologica Sinica | Cis-stilbene glucoside in Polygonum multiflorum induces immunological idiosyncratic hepatotoxicity in LPS-treated rats by suppressing PPAR-γ.[pubmed:28649126] |
| Cytokine | Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects[pubmed:29102164] |
| Molecules | Melatonin balance the autophagy and apoptosis by regulating UCP2 in the LPS-induced cardiomyopathy[Pubmed:29547569] |
| European?Journal of Pharmacology? | Naringenin ameliorates learning and memory impairment following systemic lipopolysaccharide challenge in the rat[Pubmed:29518393] |
| Regulatory Toxicology and Pharmacology | Targeted inhibition of tumor survival, metastasis and angiogenesis by Acacia ferruginea mediated regulation of VEGF, inflammatory mediators, cytokine profile and?…[Pubmed:29678767] |
| Pharmacological Reports | The Association between Inflammatory Markers (iNOS, HO-1, IL-33, MIP-1β) and Depression with and without Posttraumatic Stress Disorder[10.1016:j.pharep.2018.06.001] |
| Clinical?And Experimental Pharmacology And Physiology? | Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway[Pubmed:29266336] |
| St??enia ?ródb?onkowej i indukowalnej formy syntazy tlenku azotu w surowicy krwi kobiet ci??arnych pal?cych tytoń–badania wst?pne[] | |
| Journal of?Allergy?and?Clinical?Immunology | Exosome swarms eliminate airway pathogens and provide passive epithelial immunoprotection through nitric oxide[Pubmed: 30442371] |
| Metabolic?Brain?Disease | Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanisms[Pubmed: 30421246] |
| PLoS?One | Intraphagolysosomal conditions predispose to Staphylococcus epidermidis small colony variants persistence in macrophages[Pubmed: 30412620] |
| The?Eurasian?Journal of Medicine | Tadalafil Preserves Penile Nitric Oxide Synthase from Detrimental Effect of Paroxetine in Rats[Doi: 10.5152/eurasianjmed.2018.18160] |
| PLoS One | Macrophage expression of E3 ubiquitin ligase Grail protects mice from lipopolysaccharide-induced hyperinflammation and organ injury[Pubmed: 30571701] |
| International Journal of Environmental Research and?Public?Health | Influence of Active Exposure to Tobacco Smoke on Nitric Oxide Status of Pregnant Women[Pubmed: 30513890] |
| Journal of Functional Foods | Complex interaction of dietary fat and Alaskan bog blueberry supplementation influences manganese mediated neurotoxicity and behavioral impairments[] |
| Particle and?Fibre?Toxicology | Silica nanoparticles trigger the vascular endothelial dysfunction and prethrombotic state via miR-451 directly regulating the IL6R signaling pathway[Pubmed: 30975181] |
| ВЛИЯНИЕ ИНДУЦИБЕЛЬНОЙ NO-СИНТАЗЫ НА ТОНУС КОРОНАРНЫХ СОСУДОВ, СОКРАТИТЕЛЬНУЮ ФУНКЦИЮ МИОКАРДА И МЕХАНИЗМЫ?…[] | |
| Journal of Cosmetic Dermatology | Whitening effects of cosmetic formulation in the vascular component of skin pigmentation[Pubmed: 31074159] |
| regional hemodynamics and microcirculation | Benzimidazole Derivative, 2-ETH Prevents Poststressor Disorders of Coronary Vascular Tone and Myocardial Contractility[] |
| Life Sci | Cilostazol mitigates mesenteric ischemia/reperfusion-induced lung lesion: Contribution of PPAR-|?, NF-|êB, and STAT3 crosstalk[33310046] |
| PLoS One | Correlation analysis of the proportion of monocytic myeloid-derived suppressor cells in colorectal cancer patients[33370317] |
| Biotechnol Rep | Targeting NF-|êB mediated cell signaling pathway and inflammatory mediators by 1, 2-diazole in A549 cells in vitro[33598414] |
| Basic and Clinical Neuroscience | Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease[33995926] |
| Fermented Foods and Microbes Related to Health | Cordyceps cicadae NTTU 868 Mycelium with The Addition of Bioavailable Forms of Magnesium from Deep Ocean Water Prevents the A|?40 and Streptozotocin??-[] |
| Ya?lanma ve Hipertansiyonda Yeni bir Endoltelyal Disfonksiyon Belirteci: ??z¨1nebilir Lektin Benzeri Okside D¨1?¨1k Dansiteli Lipoprotein-1[] | |
| Fermentation | Cordyceps cicadae NTTU 868 Mycelium with The Addition of Bioavailable Forms of Magnesium from Deep Ocean Water Prevents the A|?40 and Streptozotocin-Induced Memory Deficit via Suppressing Alzheimer?ˉs Disease Risk Factors and Increasing Magnesium Uptake of Brain[] |
| Drug Chem Toxicol | Study of cardioprotective activity of the methanolic extract of the aerial parts of Bauhinia madagascariensis compared to Bauhinia purpurea against adrenaline?…[34167392] |
| Frontiers in Cardiovascular Medicine | The Effects of Intermittent Hypoxic–Hyperoxic Exposures on Lipid Profile and Inflammation in Patients With Metabolic Syndrome[34513946] |
| Nutrients | Erinacine A-Enriched Hericium erinaceus Mycelium Delays Progression of Age-Related Cognitive Decline in Senescence Accelerated Mouse Prone 8 (SAMP8) Mice[34684662] |
| biomolecules | Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men[34356664] |
| Hum Exp Toxicol | Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat[34155936] |
| J Ethnopharmacol | Post-stroke treatment of storax improves long-term outcomes of stroke in rats[34343648] |
| Phytomedicine | A multidimensional strategy for uncovering comprehensive quality markers of Schisandra chinensis (Turcz.) Baill based on pharmacodynamics and chemical?…[] |