載脂蛋白E(APOE)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Apolipoprotein E (APOE) ,etc. by FLIA (Flow Luminescence Immunoassay)
Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset
(注:單次混測多因子不超過8個指標 )
- 編號LMA704Ra
- 物種Rattus norvegicus (Rat,大鼠)相同的名稱,不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3.5h
- 檢測范圍0.39-400ng/mL
- 靈敏度最小可檢測劑量小于等于0.13 ng/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格8指標數(shù)7指標數(shù)6指標數(shù)5指標數(shù)4指標數(shù)3指標數(shù)2指標數(shù)1指標數(shù)
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特異性
本試劑盒用于檢測載脂蛋白E(APOE)等多因子檢測試劑盒(流式熒光發(fā)光法),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的載脂蛋白E(APOE)等多因子檢測試劑盒(流式熒光發(fā)光法)(加標樣品),重復(fù)測定并計算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 87-95 | 91 |
EDTA plasma(n=5) | 93-101 | 97 |
heparin plasma(n=5) | 91-101 | 97 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的載脂蛋白E(APOE)等多因子檢測試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中載脂蛋白E(APOE)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 86-99% | 96-104% | 90-101% | 92-104% |
EDTA plasma(n=5) | 97-104% | 83-101% | 92-99% | 82-102% |
heparin plasma(n=5) | 84-98% | 79-98% | 98-105% | 83-99% |
穩(wěn)定性
經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備;
2. 加樣(標準品、樣本、磁珠)標準品或樣本100μL及磁珠10μL,
37°C酶標板振蕩器孵育90分鐘;
3. 磁吸甩干,加檢測溶液A100μL,37°C酶標板振蕩器孵育60分鐘;
4. 磁吸洗板3次;
5. 加檢測溶液B100μL,37°C振動孵育30分鐘;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將載脂蛋白E(APOE)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標準品或標本以及磁珠,其中的載脂蛋白E(APOE)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的載脂蛋白E(APOE)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體,將未結(jié)合的生物素化抗體洗凈后,加入PE標記的親和素,再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的載脂蛋白E(APOE)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA704Ra02 | 載脂蛋白E(APOE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA704Ra01 | 載脂蛋白E(APOE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA704Ra01 | 載脂蛋白E(APOE)多克隆抗體 | WB; IHC; ICC; IP; FCM |
PAA704Ra02 | 載脂蛋白E(APOE)多克隆抗體 | WB; IHC; ICC; IP. |
MAA704Ra22 | 載脂蛋白E(APOE)單克隆抗體 | WB; IHC |
MAA704Ra23 | 載脂蛋白E(APOE)單克隆抗體 | WB |
MAA704Ra21 | 載脂蛋白E(APOE)單克隆抗體 | WB; IHC |
MAA704Ra24 | 載脂蛋白E(APOE)單克隆抗體 | WB; IHC |
MAA704Ra25 | 載脂蛋白E(APOE)單克隆抗體 | WB; IHC; ICC/IF |
MAA704Ra27 | 載脂蛋白E(APOE)單克隆抗體 | WB; IHC |
MAA704Ra29 | 載脂蛋白E(APOE)單克隆抗體 | IHC |
FAA704Ra02 | 抗載脂蛋白E(APOE)單克隆抗體 | FCM |
SEA704Ra | 載脂蛋白E(APOE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA704Ra | 載脂蛋白E(APOE)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA704Ra01 | 載脂蛋白E(APOE)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Seizure - European Journal of Epilepsy | Cerebrospinal fluid apolipoprotein E concentration decreases after seizure[Seizure: PIIS1059131109002441] |
Biochimica et Biophysica Acta | Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties[PubMed: 23328279 ] |
Medical Oncology | Expression and clinical significance of apolipoprotein E in pancreatic ductal adenocarcinoma[Pubmed: 23609192] |
Diabetes. | Adipocyte Atp-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth And Human Obesity[Pubmed:25249572] |
renal failure | Frequencies of apolipoprotein E alleles in depressed patients undergoing hemodialysis – a case-control study[PubMed: 25707516] |
Biochim Biophys Acta | Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism[PubMed: 26515253] |
Diabetes | Adipocyte ATP-binding cassette G1 promotes triglyceride storage, fat mass growth, and human obesity[PubMed: 25249572] |
Scientific Reports | Adaptive?immunity?against?gut?microbiota?enhances?apoE-mediated?immune?regulation?andreduces?atherosclerosis?and?western-diet-related?inflammation.[pubmed:27383250] |
Biochemical Pharmacology? | Differential effects of apoE and apoJ mimetic peptides on the action of an anti-Aβ scFv in 3xTg-AD mice[Pubmed:30026023] |
Oncotarget | Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse?…[Pubmed:29731975] |
Proteomics Clinical Applications | Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy[Pubmed: 31162828] |
Nature Communications | Hepatic HuR modulates lipid homeostasis in response to high-fat diet[Pubmed: 32546794] |
VETERINARY PATHOLOGY | Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats[Pubmed: 32880234] |
Journal of Clinical Medicine | The Significance of Apolipoprotein E Measurement in the Screening of Fetal Down Syndrome[33321701] |
Cancer Cell Int | Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer[33602229] |
Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity[34853313] |
Talanta | Iridium nanoclusters as high sensitive-tunable elemental labels for immunoassays: Determination of IgE and APOE in aqueous humor by inductively coupled?…[Pubmed:35378356] |
Analytica Chimica Acta | Gold nanoclusters as elemental label for the sequential quantification of apolipoprotein E and metallothionein 2A in individual human cells of the retinal?…[Pubmed:35361429] |