Ⅲ型膠原(COL3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Collagen Type III (COL3)
- 編號(hào)SEA176Ra
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍3.12-200ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于1.17ng/mL.
- 樣本類型serum, plasma, tissue homogenatesand other biological fluids
- 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價(jià)格¥ 2873¥ 4104¥ 18468¥ 34884¥ 287280
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實(shí)驗(yàn)結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)Ⅲ型膠原(COL3)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的Ⅲ型膠原(COL3)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 82-102 | 88 |
| EDTA plasma(n=5) | 81-93 | 87 |
| heparin plasma(n=5) | 85-99 | 88 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的Ⅲ型膠原(COL3)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中Ⅲ型膠原(COL3)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-103% | 87-101% | 81-99% | 91-101% |
| EDTA plasma(n=5) | 78-90% | 91-98% | 81-94% | 78-88% |
| heparin plasma(n=5) | 89-99% | 94-105% | 79-92% | 78-88% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將Ⅲ型膠原(COL3)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的Ⅲ型膠原(COL3)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的Ⅲ型膠原(COL3)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的Ⅲ型膠原(COL3)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| NPA176Ra01 | Ⅲ型膠原(COL3)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA176Ra01 | Ⅲ型膠原(COL3)多克隆抗體 | WB |
| SEA176Ra | Ⅲ型膠原(COL3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEA176Ra | Ⅲ型膠原(COL3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA176Ra | Ⅲ型膠原(COL3)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| nephrology dialysis transplantation | Triptolide inhibits extracellular matrix protein synthesis by suppressing the Smad2 but not the MAPK pathway in TGF-β1-stimulated NRK-49F cells[OxfordJournals: 3180] |
| Acta Otolaryngol | Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa[PubMed: 19958244] |
| Bulletin of Experimental Biology and Medicine | Modification of collagen formation by mesenchymal stem cells isolated from human adipose tissue in culture and after autotransplantation for abdominal hernia plasty[Pubmed: 24319714] |
| Age( dordr). | In vitro tenocyte metabolism in aging and oestrogen deficiency[Springer: Source] |
| Stem Cell Research Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly[Pubmed: 24739658] |
| International Journal of Advanced Research | Effect of nandrolone and/or whey protein on the soleus muscle and testis of adult male albino rats[Com:Source] |
| PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media[Pubmed:Pmc4130592] |
| Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes[Pubmed:25333747] |
| Laryngoscope. | Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria[Pubmed:25545625] |
| Cardiology | KLF15 is an essential negative regulatory factor for the cardiac remodeling response to pressure overload[Pubmed:25633973] |
| physiological research | The skin remodeling in type 1 diabetes and insulin resistance animal models[PubMed: 26047379] |
| Physiol Res | The skin remodeling in type 1 diabetes and insulin resistance animal models.[PubMed: 26047379] |
| Archives of Plastic Surgery | Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas[PubMed: 26430624] |
| Life Sci | Pituitary tumor transforming gene as a novel regulatory factor of liver fibrosis[PubMed: 25936962] |
| Molecular and Cellular Biochemistry | SVVYGLR motif of the thrombin-cleaved N-terminal osteopontin fragment enhances the synthesis of collagen type III in myocardial fibrosis[PubMed: 26112906] |
| Cardiology | KLF16 Is an Essential Negative Regulatory Factor for the Cardiac Remodeling Response to Pressure Overload[PubMed: 25633973] |
| Int J Clin Exp Med | Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-β1 with concomitant induce Smad7 in human hypertrophic scar …[PubMed: 26309506] |
| scientific reports | IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy[Pubmed:27263531] |
| Кард?олог?я | ?нфаркт м?окарда, ускладнений аневризмою л?вого шлуночка: кл?н?чн? ? структурно-функц?ональн? особливост? переб?гу, д?агностичн?, терапевтичн? та прогностичн? аспекти[bitstream:123456789] |
| International?Journal?of?Molecular?Sciences | Plasma?LncRNA-ATB, a?Potential?Biomarker?for?Diagnosis?of?Patients?with?Coal?Workers'Pneumoconiosis: A?Case-Control?Study.[pubmed:27556453] |
| BMC Cancer | Saikosaponin?d?induces?cell?death?through?caspase-3-dependent,?caspase-3-independent?andmitochondrial?pathways?in?mammalian?hepatic?stellate?cells.[pubmed:27461108] |
| Sci Rep. | Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients.[pubmed:28327551] |
| The American Journal of Chinese Medicine | Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells[pubmed:28231747] |
| Biomedical and Environmental Sciences | Crystalline Silica Promotes Rat Fibrocyte Differentiation?in Vitro, and Fibrocytes Participate in Silicosis?in Vivo[pubmed:29081339] |
| Oxidative Medicine and Cellular Longevity | Effects of the (Pro) renin Receptor on Cardiac Remodeling and Function in a Rat Alcoholic Cardiomyopathy Model via the PRR-ERK1/2-NOX4 Pathway[] |
| Cytokine | Effect of proinflammatory cytokines on endometrial collagen and metallopeptidase expression during the course of equine endometrosis[Pubmed: 31265984] |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungs[Pubmed: 32146192] |
| in vitro cellular & developmental biology-animal | Distinct differences in hypoxic responses between human oral mucosa and skin fibroblasts in a 3D collagen matrix[Pubmed: 32588253] |
| ACS Biomaterials Science & Engineering | Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction[] |
| Stem Cell Research & Therapy | Enhanced cell survival and therapeutic benefits of IL-10-expressing multipotent mesenchymal stromal cells for muscular dystrophy[33541428] |
| journal of biomedical materials research part B | Cell sheet technology: Influence of culture conditions on in vitro?\cultivated corneal stromal tissue for regenerative therapies of the ocular surface[33538123] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies[33999928] |
| BMC MOLECULAR BIOLOGY | Uncarboxylated osteocalcin alleviates the inhibitory effect of high glucose on osteogenic differentiation of mouse bone marrow¨Cderived mesenchymal stem??-[33906607] |
| Russian Journal of Cardiology | Early diagnosis of myocardial fibrosis in patients with epicardial obesity[] |
| Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction[] |
