血管內(nèi)皮生長(zhǎng)因子A(VEGFA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)
VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor
- 編號(hào)SEA143Rb
- 物種Oryctolagus cuniculus (Rabbit，兔)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍15.6-1,000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于6.5pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格48T96T96T*596T*1096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實(shí)驗(yàn)結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)血管內(nèi)皮生長(zhǎng)因子A(VEGFA)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的血管內(nèi)皮生長(zhǎng)因子A(VEGFA)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 79-94 | 89 |
| EDTA plasma(n=5) | 83-91 | 87 |
| heparin plasma(n=5) | 99-105 | 102 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的血管內(nèi)皮生長(zhǎng)因子A(VEGFA)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中血管內(nèi)皮生長(zhǎng)因子A(VEGFA)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-95% | 89-96% | 86-95% | 99-105% |
| EDTA plasma(n=5) | 83-97% | 98-105% | 98-105% | 79-103% |
| heparin plasma(n=5) | 79-89% | 87-101% | 98-105% | 96-105% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將血管內(nèi)皮生長(zhǎng)因子A(VEGFA)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的血管內(nèi)皮生長(zhǎng)因子A(VEGFA)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的血管內(nèi)皮生長(zhǎng)因子A(VEGFA)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的血管內(nèi)皮生長(zhǎng)因子A(VEGFA)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Oryctolagus cuniculus (Rabbit，兔) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA143Rb01 | 血管內(nèi)皮生長(zhǎng)因子A(VEGFA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA143Rb51 | 血管內(nèi)皮生長(zhǎng)因子A(VEGFA)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA143Rb01 | 血管內(nèi)皮生長(zhǎng)因子A(VEGFA)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA143Rb21 | 血管內(nèi)皮生長(zhǎng)因子A(VEGFA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA143Rb22 | 血管內(nèi)皮生長(zhǎng)因子A(VEGFA)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA143Rb | 血管內(nèi)皮生長(zhǎng)因子A(VEGFA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA143Rb | 血管內(nèi)皮生長(zhǎng)因子A(VEGFA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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| Acta Histochem | Paeoniflorin accelerates foot wound healing in diabetic rats though activating the Nrf2 pathway[Pubmed: 33166863] |
| Reproductive Sciences | Maternal Serum VEGF Predicts Abnormally Invasive Placenta Better than NT-proBNP: a Multicenter Case-Control Study[Pubmed: 33025531] |
| Life Sci | Circular RNA COL1A2 promotes angiogenesis via regulating miR-29b/VEGF axis in diabetic retinopathy[Pubmed: 32497630] |
| ACS NANO | Gold Nanoparticles Induce Tumor Vessel Normalization and Impair Metastasis by Inhibiting Endothelial Smad2/3 Signaling[Pubmed: 32413258] |
| Neuropsychiatric Disease and Treatment | Characterization of Endothelial Cells Associated with Cerebral Arteriovenous Malformation[Pubmed: 32368063] |
| Scientific Reports | Molecular mechanisms of synergistic action of Ramucirumab and Paclitaxel in Gastric Cancers cell lines[Pubmed: 32346056] |
| carbohydrate polymers | Effect of Chitosan oligosaccharides on ischemic symptom and gut microbiota disbalance in mice with hindlimb ischemia[Pubmed: 32475560] |
| Cutaneous and Ocular Toxicology | Isotretinoin does not alter VEGF-A and VEGF-C levels: Do retinoids behave differently in dose-dependent and/or in vivo/in vitro conditions?[Pubmed: 32722957] |
| Menoufia Medical Journal | Study of serum level of vascular endothelial growth factor in patients with hepatocellular carcinoma[] |
| Acta Scientific Women's Health | Modulatory Effects of Taurine Combined with Letrozole on Ehrlich Ascites Carcinoma in Mice[] |
| Indian Journal of Public Health Research & Development | Enhanced Fibrin-Lysis in Grade-1 Dengue Haemorrhagic Fever[] |
| Materials Science and Engineering: C | Cell-modified bioprinted microspheres for vascular regeneration[] |
| Strawberry (Fragaria x ananassa) leaf extract reduces brain damage in diabetic male rats[] | |
| Stem Cell Reviews and Reports | Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care[33469783] |
| Advanced Healthcare Materials | Accelerated Bone Regeneration by MOF Modified Multifunctional Membranes through Enhancement of Osteogenic and Angiogenic Performance[33448103] |
| Aesthet Surg J | Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water[33428732] |
| INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | In-vivo evaluation of alginate-pectin hydrogel film loaded with simvastatin for diabetic wound healing in Streptozotocin-induced diabetic rats[33421467] |
| Eur J Pharmacol | Lactucaxanthin protects retinal pigment epithelium from hyperglycemia-regulated hypoxia/ER stress/VEGF pathway mediated angiogenesis in ARPE-19 cell and rat ?-[33705802] |
| Acta Biomater | Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia[33878473] |
| Journal of Tissue Engineering | Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders[33868627] |
| EXPERIMENTAL EYE RESEARCH | Astaxanthin mediated regulation of VEGF through HIF1|á and XBP1 signaling pathway: An insight from ARPE-19 cell and streptozotocin mediated diabetic rat model[33789142] |
| Randomized Controlled Trial | Effect of Freeze-Dried Amnion Graft on Cytokines in Uterine Exudates Following Hysteroscopic Adhesiolysis of Severe Intrauterine Adhesions[28170094] |
| cancer communications | Programmed death ligand?\1 regulates angiogenesis and metastasis by participating in the c?\JUN/VEGFR2 signaling axis in ovarian cancer[33939321] |
| ?D1ú??éú3??§ó???éú3?2??ó?? | VEGFA/VEGFR2 ?úD?êó???à·????òòê×é?ˉ?a1üéú3é?Dμ?±í′??°×÷ó?[] |
| Chinese Journal of Parasitology and parasitic diseases | Expression and angiogenic effect of VEGFA/VEGFR2 in mice hepatic metacestode tissue of Echinococcus multilocularis[] |
| International Journal of Life science and Pharma Research | Artificial Sweeteners Alter Early Vitelline Vessel Development in Chick Embryo.(2021)[] |
| Biomed Mater | Preclinical efficacy study of a porous biopolymeric scaffold based on (gelatin-hyaluronic acid-chondroitin sulfate) in a porcine burn injury model: Role of critical?…[34384056] |
| J Cell Mol Med | Mesenchymal stem cells from different sources show distinct therapeutic effects in hyperoxia‐induced bronchopulmonary dysplasia in rats[34322990] |
| J Biochem Mol Toxicol | Thymoquinone upregulates miR‐125a‐5p, attenuates STAT3 activation, and potentiates doxorubicin antitumor activity in murine solid Ehrlich carcinoma[34605108] |
| Bioengineered | MicroRNA-934 facilitates cell proliferation, migration, invasion and angiogenesis in colorectal cancer by targeting B-cell translocation gene 2: Running title: miR934?…[34699325] |
| ACS Biomater Sci Eng | Polydopamine-Coated Poly(l-lactide) Nanofibers with Controlled Release of VEGF and BMP-2 as a Regenerative Periosteum[34472855] |
| Cells | A-Kinase Anchoring Protein 2 Promotes Protection against Myocardial Infarction[34831084] |
| J Basic Clin Physiol Pharmacol | Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model rats[Pubmed:34986543] |
| Stem Cell Res Ther | A dual role of HIF1α in regulating osteogenesis–angiogenesis coupling[Pubmed:35123567] |
| American Journal of Translational Research | Curcumin treats endometriosis in mice by the HIF signaling pathway[Pubmed:35559378] |
| Clinical and Experimental Immunology | Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patients[Pubmed:35348620] |
| Medicina | Assessment of the RANTES Level Correlation and Selected Inflammatory and Pro-Angiogenic Molecules Evaluation of Their Influence on CRC Clinical Features: A?…[Pubmed:35208526] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?…[Pubmed:35585377] |
| Biomaterials Advances | Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats[] |
| Biointerface Research in Applied Chemistry | Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats[] |
| Research Square | DMOG attenuates steroid-associated endothelial progenitor cell impairment and osteonecrosis of the femoral head via regulating HIF-1α signaling pathway in?…[] |
