攝食抑制因子1(NES1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Nesfatin 1 (NES1)
產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實(shí)驗(yàn)結(jié)果圖
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)攝食抑制因子1(NES1)，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的攝食抑制因子1(NES1)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 85-92 | 89 |
| EDTA plasma(n=5) | 79-101 | 85 |
| heparin plasma(n=5) | 97-104 | 101 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的攝食抑制因子1(NES1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中攝食抑制因子1(NES1)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-99% | 79-93% | 92-101% | 79-104% |
| EDTA plasma(n=5) | 91-99% | 91-105% | 89-103% | 78-95% |
| heparin plasma(n=5) | 83-101% | 98-105% | 78-95% | 99-105% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將攝食抑制因子1(NES1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的攝食抑制因子1(NES1)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的攝食抑制因子1(NES1)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的攝食抑制因子1(NES1)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA242Mu01 | 攝食抑制因子1(NES1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA242Mu01 | 攝食抑制因子1(NES1)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA242Mu01 | 攝食抑制因子1(NES1)多克隆抗體 | WB; IHC |
| SEA242Mu | 攝食抑制因子1(NES1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA242Mu | 攝食抑制因子1(NES1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA242Mu | 攝食抑制因子1(NES1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA242Mu | 攝食抑制因子1(NES1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Endocrine | Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitus[SpringerLink: h978088657555w16] |
| Bulletin of Clinical Psychopharmacology | Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder [Psikofarmakoloji: 2212] |
| Psychoneuroendocrinology. | Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorder[PubMed: 22595767] |
| Iranian Journal of Health and Physical activity | Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female Rats[Ijvst: Source] |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Decreased serum nesfatin-1 levels in endometriosis[Pubmed: 24702902] |
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| An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages[] | |
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| Ann Nutr Metab | Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity[Pubmed: 33027789] |
| Endokrynologia Polska | Assessing the blood concentration of new adipocytokines in patients with ischaemic stroke[33283259] |
| Increased serum nesfatin-1 levels among adolescents diagnosed with major depressive disorder[] |
