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纖溶酶原(Plg)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

編號KSB236Ra01
物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
試劑內(nèi)容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
可檢測范圍15.6-1,000ng/mL
可適靈敏度6.9ng/mL
應用Main materials for "Do It (ELISA Kit) Yourself".
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規(guī)格96T*596T*1096T*2096T*5096T*100
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試劑盒中的抗體對纖溶酶原(Plg)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。

抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。

編號	適用物種：Rattus norvegicus (Rat，大鼠)	應用(僅供研究使用，不用于臨床診斷！)
RPB236Ra01	纖溶酶原(Plg)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
APB236Ra01	纖溶酶原(Plg)活性蛋白	Cell?culture;?Activity?Assays.
PAB236Ra01	纖溶酶原(Plg)多克隆抗體	WB; IHC; ICC; IP.
FAB236Ra01	抗纖溶酶原(Plg)多克隆抗體	FCM
MAB236Ra21	纖溶酶原(Plg)單克隆抗體	WB
MAB236Ra22	纖溶酶原(Plg)單克隆抗體	WB; IHC; ICC; IP.
SEB236Ra	纖溶酶原(Plg)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB236Ra	纖溶酶原(Plg)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
KSB236Ra01	纖溶酶原(Plg)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)	Main materials for "Do It (ELISA Kit) Yourself".

雜志	參考文獻
International Journal of Molecular Sciences	The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC[Mdpi: Source]
J Anim Physiol Anim Nutr (Berl).	Improved milk production through PG-PL system by provision of in-house shelter management in lactating Murrah buffaloes during winter season.[pubmed:28084661]
Neurotherapeutics	iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management[pubmed:29247448]
Veterinarski?Arhiv	The influence of a modified micro-environment on stress and milk production through the plasminogen-plasmin system in Murrah buffaloes during the hot-humid?…[10.24099:vet.arhiv.170113]
Food Bioscience	Reducing antigenicity of \|?-lactoglobulin, probiotic properties and safety evaluation of Lactobacillus plantarum AHQ-14 and Lactobacillus bulgaricus BD0390[]
Foods	Antigenicity and Safety Evaluation of Lactiplantibacillus plantarum 7-2 Screened to Reduce α-Casein Antigen[Pubmed:35010214]