攝食抑制因子1(NES1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Nesfatin 1 (NES1)
- 編號KSA242Hu11
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma and other biological fluids.
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍617.3-50000pg/mL
- 可適靈敏度251.4pg/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對攝食抑制因子1(NES1)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA242Hu02 | 攝食抑制因子1(NES1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA242Hu03 | 攝食抑制因子1(NES1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA242Hu01 | 攝食抑制因子1(NES1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA242Hu21 | 攝食抑制因子1(NES1)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA242Hu22 | 攝食抑制因子1(NES1)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA242Hu02 | 攝食抑制因子1(NES1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA242Hu08 | 攝食抑制因子1(NES1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA242Hu01 | 攝食抑制因子1(NES1)多克隆抗體 | WB; IHC; ICC/IF |
| SEA242Hu | 攝食抑制因子1(NES1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA242Hu | 攝食抑制因子1(NES1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA242Hu | 攝食抑制因子1(NES1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA242Hu11 | 攝食抑制因子1(NES1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Bulletin of Clinical Psychopharmacology | Plasma Nesfatin-1 Level May Be Associated with Disease Severity in Patients with Panic Disorder[Psikofarmakoloji: 2043] |
| Progress in Neuro-Psychopharmacology & Biological Psychiatry | High plasma nesfatin-1 level in patients with major depressive disorder[PubMed: 21163317] |
| Endocrine | Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitus[SpringerLink: h978088657555w16] |
| Bulletin of Clinical Psychopharmacology | Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder [Psikofarmakoloji: 2212] |
| Psychoneuroendocrinology. | Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorder[PubMed: 22595767] |
| Iranian Journal of Health and Physical activity | Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female Rats[Ijvst: Source] |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Decreased serum nesfatin-1 levels in endometriosis[Pubmed: 24702902] |
| Bulletin of Clinical Psychopharmacology | Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder.[Psikofarmakoloji: Source] |
| Current Pharmaceutical Design | Role of NUCB2/nesfatin-1 as a possible biomarker.[Pubmed: 23537082] |
| Regulatory, Integrative and Comparative Physiology | A role of nesfatin-1/NucB2 in dehydration-induced anorexia[Pubmed:24829503] |
| J Endocrinol Invest. | Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndrome[Pubmed:24920281] |
| Int J Surg. | The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study.[Pubmed:25448651] |
| Hormone molecular biology and clinical investigation | The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markers[Pubmed:25581765] |
| Turk J Gastroenterol. | New hormones to predict the severity of gallstone-induced acute pancreatitis[Pubmed:25599787] |
| Respiration | Is There a Relationship between Obstructive Sleep Apnea Syndrome Severity and Nesfatin-1?[PubMed: 26088934] |
| J Clin Res Pediatr Endocninol | Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished Children[PubMed: 26831553] |
| Bosn J Basic Med Sci | Nesfatin-1 and Vitamin D levels may be associated with systolic and diastolic blood pressure values and hearth rate in polycystic ovary syndrome[PubMed: 26295295] |
| Original Article / Orijinal Makale | Relationship and Interaction between Anaerobic Sports Branches and Serum Nesfatin-1[Sayilar: 294] |
| Gynecol Endocrinol | Nesfatin-1 levels and metabolic markers in polycystic ovary syndrome[PubMed: 26062107] |
| International Journal of Neuroscience | Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage*[Pubmed:26872406] |
| 未找到 | Comparing the nesfatin-1 levels between individuals with subjective cognitive decline and mild cognitive impairment[P.1.e.012.pdf] |
| International Journal of Developmental | Serum?nesfatin-1?levels: a?potential?new?biomarker?in?patients?with?subarachnoid?hemorrhage.[pubmed:26872406] |
| Current Pharmaceutical Design | Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Disease[pubmed:28137218] |
| Arch Endocrinol Metab | Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetes[pubmed:28977160] |
| Indian Journal of Psychiatry | Serum NUCB2/nesfatin-1 levels in different stages of alcohol dependence: Is there a relationship with craving?[pubmed:28529367] |
| Biomedicine & Pharmacotherapy | Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ[Pubmed:29604590] |
| Biochimica et?Biophysica?Acta | Nesfatin-1 functions as a switch for phenotype transformation and proliferation of VSMCs in hypertensive vascular remodeling[Pubmed:29627363] |
| BMC Psychiatry | Plasma nesfatin-1 level is associated with severity of depression in Chinese depressive patients[Pubmed:29615007] |
| Probiotics?and Antimicrobial?Proteins | Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad Shidfar[Pubmed: 30232744] |
| An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages[] | |
| clinical neurology and neurosurgery | Relationsh?p of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels[Pubmed: 32311618] |
| Ann Nutr Metab | Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity[Pubmed: 33027789] |
| Endokrynologia Polska | Assessing the blood concentration of new adipocytokines in patients with ischaemic stroke[33283259] |
| Increased serum nesfatin-1 levels among adolescents diagnosed with major depressive disorder[] |