白介素31(IL31)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Interleukin 31 (IL31)
- 編號KSB179Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 試劑內容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍15.6-1,000pg/mL
- 可適靈敏度6.7pg/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產品包裝(模擬)
產品包裝(模擬)
通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對白介素31(IL31)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| EPB179Ra61 | 白介素31(IL31)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB179Ra01 | 白介素31(IL31)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB179Ra01 | 白介素31(IL31)多克隆抗體 | WB |
| LAB179Ra81 | 白介素31(IL31)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAB179Ra71 | 白介素31(IL31)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAB179Ra21 | 白介素31(IL31)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB179Ra | 白介素31(IL31)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB179Ra | 白介素31(IL31)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB179Ra01 | 白介素31(IL31)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| allergy and clinical immunology | Increased serum levels of Interleukin-31 could modulate inflammatory processes in multiple sclerosis[Siaic-Review:Source] |
| BMC Immunol | Increased levels of interleukin 31 (IL-31) in osteoporosis[PubMed: 2644965] |
| Sci Rep. | Interleukin-31 expression and relation to disease severity in human asthma[pmc:PMC4783779] |
| Exp Dermatol. | Immediate-type allergic and protease-mediated reactions are involved in scratching behaviour induced by topical application of Dermatophagoides farinae extract in NC/Nga mice.[pubmed:28191683] |
| Nat Commun.? | The transcription factor EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction.[pubmed:28067314] |
| Experimental Dermatology | Immediate-type allergic and protease-media ted reactions are involved in scratching behavior induced by topical application of Dermatophagoides farinae extract in NC/Nga mice[doi:10.1111] |
| アトピー性皮膚炎の癢みの発癥機序に関する研究[] | |
| Indian Journal of Public Health Research & Development | Determination the Role of Interleukin 31 (IL-31) Levels in Three Real Allergic Diseases (Asthma, Rhinitis, Urticaria)[] |