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促性腺激素釋放激素(GnRH)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Gonadotropin Releasing Hormone (GnRH)

GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1

編號(hào)KSA843Mu11
物種Mus musculus (Mouse，小鼠)相同的名稱(chēng)，不同的物種。
試劑內(nèi)容包被抗體、生物素標(biāo)記的競(jìng)爭(zhēng)物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
可適用原理競(jìng)爭(zhēng)抑制酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
可檢測(cè)范圍12.35-1000pg/mL
可適靈敏度4.33pg/mL
應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
規(guī)格96T*596T*1096T*2096T*5096T*100
價(jià)格¥ 699811664¥ 11664¥ 20995¥ 40824¥ 69984
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)促性腺激素釋放激素(GnRH)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。

配套試劑

贈(zèng)品

ELISA/CLIA實(shí)驗(yàn)服務(wù)

增值服務(wù)

化學(xué)發(fā)光試劑盒定制服務(wù)
酶聯(lián)免疫試劑盒定制服務(wù)
檢測(cè)試劑盒DIY輔助試劑包1
檢測(cè)試劑盒DIY輔助試劑包2
標(biāo)準(zhǔn)品稀釋液
 包被緩沖液
 抗體稀釋緩沖液
 檢測(cè)試劑盒DIY輔助試劑包3
檢測(cè)試劑盒DIY輔助試劑包4
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

相關(guān)產(chǎn)品

編號(hào)	適用物種：Mus musculus (Mouse，小鼠)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA843Mu01	促性腺激素釋放激素(GnRH)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPA843Mu02	促性腺激素釋放激素(GnRH)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
SPA843Mu01	促性腺激素釋放激素(GnRH)合成多肽	Immunogen; Blocking Peptide.
PAA843Mu01	促性腺激素釋放激素(GnRH)多克隆抗體	WB
PAA843Mu02	促性腺激素釋放激素(GnRH)多克隆抗體	WB; IHC; ICC; IP.
LAA843Mu71	促性腺激素釋放激素(GnRH)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
LAA843Mu81	促性腺激素釋放激素(GnRH)多克隆抗體(異硫氰酸熒光素標(biāo)記)	WB; IHC; ICC; IF.
CEA843Mu	促性腺激素釋放激素(GnRH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
AEA843Mu	抗促性腺激素釋放激素抗體(Anti-GnRH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antibody Detection.
CCA843Mu	促性腺激素釋放激素(GnRH)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
LMA843Mu	促性腺激素釋放激素(GnRH)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
KSA843Mu11	促性腺激素釋放激素(GnRH)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Mol Pharmacol.	Maternal exposure to dioxin imprints sexual immaturity of the pups through fixing the status of the reduced expression of hypothalamic gonadotropin-releasing …[Pubmed:24132183]
Journal of Sports Science & Medicine	Heavy Resistance Training and Supplementation With the Alleged Testosterone Booster Nmda has No Effect on Body Composition, Muscle Performance, and Serum Hormones Associated With the Hypothalamo-Pituitary-Gonadal Axis in Resistance-Trained Males[Pubmed:24570624]
Environmental Research	Perfluorooctane sulfonate effects on the reproductive axis in adult male rats[Pubmed:25171141]
Toxicology Letters	Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate[Pubmed:25623392]
Toxicol Sci	Chronic exposure of female mice to an environmental level of perfluorooctane sulfonate suppresses estrogen synthesis through reduced histone H3K14 acetylation of the StAR promoter leading to deficits in follicular development and ovulation[PubMed: 26358002]
Int J Mol Sci.	Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice[Pubmed:26771609]
Oral Presentations	OP0019-HPR Effectiveness of A Progressive Resistance Strength Program on Hand Osteoarthritis: A Randomised Controlled Trial[60.1.abstract]
Oral Presentations	OP0021-HPR Sleep Disorders in Patients with Rheumatoid Arthritis and Relationship with Disease Activity[60.3.abstract]
Toxicological?Sciences	Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring[pubmed:27803384]
Chronobiology of the immune reponse	OP0020?The Association of Gonadotropin-Releasing Hormone and Cytokines in Rheumatoid Arthritis[content:75]
Environmental Science & Technology	Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis[pubmed:28731686]
Toxicological?Sciences	Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons[10.1093:toxsci]
GENES	Nectin-like molecule 2, a necessary sexual maturation regulator, participates in congenital hypogonadotropic hypogonadism[Pubmed: 32535046]
BioMed Research International	Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress[Pubmed: 32685488]
JOURNAL OF ETHNOPHARMACOLOGY	Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis[33422655]
J Virol	Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice[34730391]