載脂蛋白E(APOE)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Apolipoprotein E (APOE)
Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset
- 編號KSA704Po01
- 物種Sus scrofa; Porcine (Pig，豬)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.312-20ng/mL
- 可適靈敏度0.142ng/mL
- 應(yīng)用Main materials for "Do It(ELISA Kit) Yourself"
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對載脂蛋白E(APOE)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Sus scrofa; Porcine (Pig，豬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA704Po01 | 載脂蛋白E(APOE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA704Po01 | 載脂蛋白E(APOE)多克隆抗體 | WB,IHC |
| MAA704Po21 | 載脂蛋白E(APOE)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA704Po | 載脂蛋白E(APOE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA704Po | 載脂蛋白E(APOE)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA704Po01 | 載脂蛋白E(APOE)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻
| 雜志 | 參考文獻 |
| Seizure - European Journal of Epilepsy | Cerebrospinal fluid apolipoprotein E concentration decreases after seizure[Seizure: PIIS1059131109002441] |
| Biochimica et Biophysica Acta | Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties[PubMed: 23328279 ] |
| Medical Oncology | Expression and clinical significance of apolipoprotein E in pancreatic ductal adenocarcinoma[Pubmed: 23609192] |
| Diabetes. | Adipocyte Atp-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth And Human Obesity[Pubmed:25249572] |
| renal failure | Frequencies of apolipoprotein E alleles in depressed patients undergoing hemodialysis – a case-control study[PubMed: 25707516] |
| Biochim Biophys Acta | Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism[PubMed: 26515253] |
| Diabetes | Adipocyte ATP-binding cassette G1 promotes triglyceride storage, fat mass growth, and human obesity[PubMed: 25249572] |
| Scientific Reports | Adaptive?immunity?against?gut?microbiota?enhances?apoE-mediated?immune?regulation?andreduces?atherosclerosis?and?western-diet-related?inflammation.[pubmed:27383250] |
| Biochemical Pharmacology? | Differential effects of apoE and apoJ mimetic peptides on the action of an anti-Aβ scFv in 3xTg-AD mice[Pubmed:30026023] |
| Oncotarget | Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse?…[Pubmed:29731975] |
| Proteomics Clinical Applications | Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy[Pubmed: 31162828] |
| Nature Communications | Hepatic HuR modulates lipid homeostasis in response to high-fat diet[Pubmed: 32546794] |
| VETERINARY PATHOLOGY | Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats[Pubmed: 32880234] |
| Journal of Clinical Medicine | The Significance of Apolipoprotein E Measurement in the Screening of Fetal Down Syndrome[33321701] |
| Cancer Cell Int | Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer[33602229] |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity[34853313] |
| Talanta | Iridium nanoclusters as high sensitive-tunable elemental labels for immunoassays: Determination of IgE and APOE in aqueous humor by inductively coupled?…[Pubmed:35378356] |
| Analytica Chimica Acta | Gold nanoclusters as elemental label for the sequential quantification of apolipoprotein E and metallothionein 2A in individual human cells of the retinal?…[Pubmed:35361429] |