類胰蛋白酶(TPS)檢測試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Tryptase (TPS)
TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III
- 編號(hào)SCB070Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長2h, 40min
- 檢測范圍1.37-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于0.49pg/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實(shí)驗(yàn)結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測類胰蛋白酶(TPS)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的類胰蛋白酶(TPS)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 96-103 | 101 |
| EDTA plasma(n=5) | 87-104 | 94 |
| heparin plasma(n=5) | 97-105 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的類胰蛋白酶(TPS)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中類胰蛋白酶(TPS)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-89% | 97-105% | 78-99% | 95-103% |
| EDTA plasma(n=5) | 80-99% | 79-91% | 78-89% | 97-105% |
| heparin plasma(n=5) | 90-98% | 80-90% | 96-103% | 83-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實(shí)驗(yàn)原理
將類胰蛋白酶(TPS)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的類胰蛋白酶(TPS)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的類胰蛋白酶(TPS)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入底物。加入底物后會(huì)產(chǎn)生輝光型光信號(hào)。發(fā)射光強(qiáng)度和樣品中的類胰蛋白酶(TPS)呈正相關(guān)。用化學(xué)發(fā)光儀測定相對(duì)光單位（RLU），計(jì)算樣品濃度。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB070Hu02 | 類胰蛋白酶(TPS)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB070Hu01 | 類胰蛋白酶(TPS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB070Hu02 | 類胰蛋白酶(TPS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB070Hu01 | 類胰蛋白酶(TPS)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB070Hu71 | 類胰蛋白酶(TPS)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB070Hu21 | 類胰蛋白酶(TPS)單克隆抗體 | IHC |
| MAB070Hu24 | 類胰蛋白酶(TPS)單克隆抗體 | WB; IHC |
| MAB070Hu26 | 類胰蛋白酶(TPS)單克隆抗體 | WB; IHC |
| MAB070Hu22 | 類胰蛋白酶(TPS)單克隆抗體 | WB; IHC |
| MAB070Hu23 | 類胰蛋白酶(TPS)單克隆抗體 | WB; IHC |
| LAB070Hu76 | 類胰蛋白酶(TPS)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| FAB070Hu86 | 抗類胰蛋白酶(TPS)單克隆抗體(異硫氰酸熒光素標(biāo)記) | FCM |
| WEB070Hu | 類胰蛋白酶(TPS)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEB070Hu | 類胰蛋白酶(TPS)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB070Hu | 類胰蛋白酶(TPS)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB070Hu | 類胰蛋白酶(TPS)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSB070Hu01 | 類胰蛋白酶(TPS)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSB070Hu01 | 類胰蛋白酶(TPS)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| J Pediatr. | Improved liver function and relieved pruritus after 4-phenylbutyrate therapy in a patient with progressive familial intrahepatic cholestasis type 2[Pubmed:24530123] |
| Mediators of Inflammation | Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis[Pubmed:25400334] |
| PLOS ONE | A Novel Model of IgE-Mediated Passive Pulmonary Anaphylaxis in Rats[Pubmed:25541997] |
| Neurogastroenterology & Motility | Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgery[Pubmed:25677271] |
| Mediators Inflamm | Elevated Serum Tryptase and Endothelin in Patients with ST Segment Elevation Myocardial Infarction: Preliminary Report[PubMed: 26089601] |
| Int Immunopharmacol | Tryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitis[PubMed: 26049029] |
| Mediators of Inflammation | Altered expression of IFN-λ2 in allergic airway disorders and identification of its cell origins[Journals: Mi] |
| Mediators of Inflammation | Altered Expression of IFN-2 in Allergic Airway Disorders and Identification of Its Cell Origins[Pubmed:27057098] |
| Analytica Chimica Acta | An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis[] |
| Journal of Clinical Investigation | Dengue virus-elicited tryptase induces endothelial permeability and shock[Pubmed: 31265436] |
| Frontiers in Physiology | Mast Cells in Peritoneal Fluid From Women With Endometriosis and Their Possible Role in Modulating Sperm Function[Pubmed: 31998139] |
| Biomarkers of Allergic Asthma and their Association with Serum Parameters[] | |
| Annals of Tropical Medicine & Public Health | Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity[] |
| Front Immunol | Serum CD203c+ Extracellular Vesicle Serves as a Novel Diagnostic and Prognostic Biomarker for Succinylated Gelatin Induced Perioperative Hypersensitive?…[34650557] |
| Microbiol Spectr | Mast Cell-Derived Proteases Induce Endothelial Permeability and Vascular Damage in Severe Fever with Thrombocytopenia Syndrome[Pubmed:35612327] |