神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)檢測試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Glial Fibrillary Acidic Protein (GFAP)
Intermediate Filament Protein
- 編號SCA068Ra
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長2h, 40min
- 檢測范圍4.12-3,000pg/mL
- 靈敏度最小可檢測劑量小于等于1.41pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 3830¥ 5472¥ 24624¥ 46512¥ 383040
- 欲了解實際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結(jié)果圖
標(biāo)準曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 84-91 | 87 |
| EDTA plasma(n=5) | 81-91 | 85 |
| heparin plasma(n=5) | 94-103 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-99% | 96-104% | 95-103% | 97-104% |
| EDTA plasma(n=5) | 87-94% | 89-96% | 93-101% | 83-94% |
| heparin plasma(n=5) | 81-94% | 80-99% | 86-102% | 86-95% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準品、試劑及樣本的準備；
2. 加樣（標(biāo)準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實驗原理
將神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準品或標(biāo)本，其中的神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)呈正相關(guān)。用化學(xué)發(fā)光儀測定相對光單位（RLU），計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
參考文獻
| 雜志 | 參考文獻 |
| Journal of Occupational Health | Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat Brain[Pubmed: 23183024] |
| Morfovirtual 2014 | EFECTO PROTECTOR DEL TRATAMIENTO CON NEUROEPO SOBRE LA GLIA DE LA CORTEZA CEREBRAL EN RATONES TRANSGéNICOS CON LA ENFERMEDAD DE ALZHEIMER.[Sld:Source] |
| Annals of Anatomy | Effect of memantine, a NMDA receptor blocker, on ethambutol-induced retinal injury[PubMed: 26704355] |
| Mediators of Inflammation | IL-4 Inhibits IL-1β-Induced Depressive-Like Behavior and Central Neurotransmitter Alterations[PubMed: 26417153] |
| Neurochem Res | Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the Rat[PubMed: 26700436] |
| Cell Mol Neurobiol | Hypericum Perforatum Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s Disease[PubMed: 26119304] |
| Sleep and Biological Rhythms | Relationship of orexin (hypocretin) system and astrocyte activation in Parkinson's disease with hypersomnolence[Doi: 10.1111] |
| Cellular and Molecular Neurobiology | Hypericum Perforatum?Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s Disease[Pubmed:26119304] |
| Ann Anat | Effect of memantine: A NMDA receptor blocker, on ethambutol-induced retinal injury[Pubmed:26704355] |
| United States Patent Application | ESTROGENIC COMPONENTS FOR USE IN THE TREATMENT OF NEUROLOGICAL DISORDERS[y2016:0101116.html] |
| Clinical Research | Human plasma glial fibrillary acidic protein and heat shock protein 27 concentrations in acute moderate-intensity aerobic exercise with different duration[a825e1e27b0221df9a627769c0f614432e92.pdf] |
| Medical Journal of Indonesia | Comparison of GFAP and HSP27 concentrations in acute moderate-intensity aerobic exercise of different duration[2031884] |
| Peptides. | Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms.[pubmed:27777064] |
| european journal of pharmacology | Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms.[pubmed:27915041] |
| european journal of pharmacology | Troxerutin exerts neuroprotection in 6-hydroxydopamine lesion rat model of Parkinson's disease: Possible involvement of PI3K/ERβ signaling.[pubmed:28284752] |
| biomedicine & pharmacotherapy | Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat.[pubmed:28199883] |
| J Endocrinol.? | Estrogen receptors and estetrol-dependent neuroprotective actions: a pilot study.[pubmed:27799463] |
| IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's …[articles:journal-of-alzheimers-disease] |
| Tier?rztliche Hochschule Hannover | Evaluierung von Biomarkern im Liquor cerebrospinalis bei paraplegischen Hunden mit Ru?ckenmarksverletzung[:] |
| Nigerian Journal of Clinical Practice | Utilization of Glial Fibrillary Acidic Protein and Galectin-3 in the Diagnosis of Cerebral Infarction Patients with Normal Cranial Tomography[pubmed:28406123] |
| Cytokine | Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects[pubmed:29102164] |
| Biomedicine & pharmacotherapy | Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms[pubmed:28061403] |
| Experimental gerontology | The anti-aging protein klotho alleviates injury of nigrostriatal dopaminergic pathway in 6-hydroxydopamine rat model of Parkinson's disease: Involvement of PKA/CaMKII/CREB signaling.[pubmed:29107062] |
| Pesticide Biochemistry and Physiology | Acrolein-mediated neurotoxicity in growing Wistar male rats[Pubmed:30033014] |
| Journal of Perinatal Medicine | Mechanisms of death in structurally normal stillbirths[Pubmed: 30231013] |
| Metabolic?Brain?Disease | Berberine ameliorates lipopolysaccharide-induced learning and memory deficit in the rat: insights into underlying molecular mechanisms[Pubmed: 30456649] |
| Metabolic?Brain?Disease | Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanisms[Pubmed: 30421246] |
| 博士論文 | INSTITUTO DE CIENCIAS BáSICAS Y PRECLíNICAS “VICTORIA DE GIRóN” DEPARTAMENTO DE HISTOLOGíA[] |
| Medicina | The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the?…[] |
| Open Access Macedonian Journal of Medical Sciences | Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic Retinopathy[Pubmed: 32165929] |
| NEUROPSYCHOBIOLOGY | Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying Mechanisms[Pubmed: 32526752] |
| JAMA Surgery | Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairment[Pubmed: 28593326] |
| Acta Histochemica | Quercetin mitigates monosodium glutamate-induced excitotoxicity of the spinal cord motoneurons in aged rats via p38 MAPK inhibition[Pubmed: 32622428] |
| stanbul Med J | The Predictive Role of Neurobiochemical Markers in Multiple Sclerosis.[] |
| Basic and Clinical Neuroscience | Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease[33995926] |
| pharmacology online | PHARMACOLOGICAL SCREENING OF A POTENTIALLY EFFECTIVE COMPOUND FOR THE TREATMENT OF CHRONIC TRAUMATIC ENCEPHALOPATHY??-[] |
| Neuropeptides | The bee venom active compound melittin protects against bicuculline-induced seizures and hippocampal astrocyte activation in rats[34808488] |
| Neural Plast | P2Y2 Receptor Mediated Neuronal Regeneration and Angiogenesis to Affect Functional Recovery in Rats with Spinal Cord Injury[Pubmed:35154311] |