骨成型蛋白2(BMP2)檢測試劑盒(化學發(fā)光免疫分析法)
CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)
BMP2A; BMP-2A; Hemochromatosis Modifier
- 編號SCA013Rb
- 物種Oryctolagus cuniculus (Rabbit，兔)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長2h, 40min
- 檢測范圍13.7-10,000pg/mL
- 靈敏度最小可檢測劑量小于等于6.4pg/mL.
- 樣本類型Serum, plasma, tissue homogenates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 3830¥ 5472¥ 24624¥ 46512¥ 383040
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結(jié)果圖
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測骨成型蛋白2(BMP2)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的骨成型蛋白2(BMP2)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 96-104 | 101 |
| EDTA plasma(n=5) | 79-101 | 89 |
| heparin plasma(n=5) | 87-101 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的骨成型蛋白2(BMP2)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中骨成型蛋白2(BMP2)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 90-103% | 95-103% | 91-99% |
| EDTA plasma(n=5) | 96-103% | 80-101% | 98-105% | 92-105% |
| heparin plasma(n=5) | 87-94% | 96-104% | 87-95% | 80-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實驗原理
將骨成型蛋白2(BMP2)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的骨成型蛋白2(BMP2)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的骨成型蛋白2(BMP2)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的骨成型蛋白2(BMP2)呈正相關(guān)。用化學發(fā)光儀測定相對光單位（RLU），計算樣品濃度。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Oryctolagus cuniculus (Rabbit，兔) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA013Rb02 | 骨成型蛋白2(BMP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA013Rb01 | 骨成型蛋白2(BMP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA013Rb51 | 骨成型蛋白2(BMP2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA013Rb01 | 骨成型蛋白2(BMP2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA013Rb52 | 骨成型蛋白2(BMP2)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA013Rb71 | 骨成型蛋白2(BMP2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEA013Rb | 骨成型蛋白2(BMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA013Rb | 骨成型蛋白2(BMP2)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA013Rb | 骨成型蛋白2(BMP2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| Medical Journal of Chinese People's Liberation Army | Effect of transplantation of BMP-2-induced bone marrow mesenchymal stem cells on myocardial infarction of rats after reperfusion[Source] |
| PLOS ONE | PDGF-AA promotes osteogenic differentiation and migration of mesenchymal stem cell by down-regulating PDGFRα and derepressing BMP-Smad1/5/8 signaling[Pubmed:25470749] |
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| Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats[Pubmed:29898457] |
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| ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | The dose-time effects of fluoride on the expression and DNA methylation level of the promoter region of BMP-2 and BMP-7 in rats[Pubmed: 32004919] |
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