Smad同源物3(SMAD3)單克隆抗體
Monoclonal Antibody to SMAD family member 3 (SMAD3)
JV15-2; MADH3; Mothers Against Decapentaplegic Homolog 3
- 編號MAC123Hu22
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C4
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原RPC123Hu01-Smad同源物3(SMAD3)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/ml
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應用WB; IHC
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 666¥ 1554¥ 2220¥ 5550¥ 22200
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Sample: Human Cerebrum Tissue; Primary Ab: 40μg/ml Mouse Anti-Human Smad3 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 40μg/ml Mouse Anti-Human Smad3 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 40μg/ml Mouse Anti-Human Smad3 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Porcine Pancreas lysate Primary Ab: 0.2μg/ml Mouse Anti-Human SMAD3 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: Porcine Pancreas lysate; Lane2: Jurkat cell lysate; Lane3: 293T cell lysate
Primary Ab: 2μg/ml Mouse Anti-Human Smad3 Antibody
Second Ab: 0.2μg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對SMAD3的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別SMAD3。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPC123Hu01 | Smad同源物3(SMAD3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC123Hu01 | Smad同源物3(SMAD3)多克隆抗體 | WB |
| FAC123Hu01 | 抗Smad同源物3(SMAD3)多克隆抗體 | FCM |
| MAC123Hu21 | Smad同源物3(SMAD3)單克隆抗體 | WB; IHC |
| MAC123Hu22 | Smad同源物3(SMAD3)單克隆抗體 | WB; IHC |
| MAC123Hu23 | Smad同源物3(SMAD3)單克隆抗體 | WB; IHC |
參考文獻
| 雜志 | 參考文獻 |
| D￡?ˉ | Role of Angiotensin II and Renal Nerves on TGF-b/SmadPathway in Diabetic Nephropathy Rat Model[] |