胰島素樣生長因子結(jié)合蛋白1(IGFBP1)單克隆抗體
Monoclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1)
IBP1; AFBP; IGF-BP25; PP12; Placental Protein 12; Amniotic Fluid Binding Protein; Alpha-Pregnancy-Associated Endometrial Globulin; Growth Hormone Independent-Binding Protein
- 編號MAA052Hu22
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)D9
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原RPA052Hu02-胰島素樣生長因子結(jié)合蛋白1(IGFBP1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/ml
- 且適物種Rattus norvegicus (Rat，大鼠)
- 應用WB; IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 634¥ 1478¥ 2112¥ 5280¥ 21120
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Sample: Human Spleen Tissue; Primary Ab: 30μg/ml Mouse Anti-Human IGFBP1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Spleen Tissue
Primary Ab: 30μg/ml Mouse Anti-Human IGFBP1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Figure. Western Blot; Sample: Recombinant IGFBP1, Human.
Western Blot; Sample: Lane1: Human Placenta lysate; Lane2: Rat Placenta lysate<br/>Primary Ab: 2μg/ml Mouse Anti-Human IGFBP1 Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對IGFBP1的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別IGFBP1。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
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參考文獻
| 雜志 | 參考文獻 |
| Endocrinology | Lack of Dietary Carbohydrates Induces Hepatic Growth Hormone (GH) Resistance in Rats[Endo: source] |
| Endocrinology | Serum IGF-I Is Not a Reliable Pharmacodynamic Marker of Exogenous Growth Hormone Activity in Mice[Endo: source] |
| Animal. | Effects of a dietary crude fibre concentrate on growth in weaned piglets.[pubmed:28318464] |
| Stem Cell Research & Therapy | Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cells[pubmed:29096715] |