芳香烴受體(AhR)多克隆抗體
Polyclonal Antibody to Aryl Hydrocarbon Receptor (AhR)
BHLHE76; Class E basic helix-loop-helix protein 76
- 編號PAB354Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPB354Hu01-芳香烴受體(AhR)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 598¥ 1394¥ 1992¥ 4980¥ 19920
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Sample: Mouse Cerebrum Tissue Primary Ab: 10μg/ml Rabbit Anti-Human AhR Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human AhR Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20μg/ml Rabbit Anti-Human AhR Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
Figure. Western Blot; Sample: Recombinant AhR, Human.
Western Blot; Sample: Lane1: MCF7 cell lysate; Lane2: 293T cell lysate; Lane3: 3T3-L1 cell lysate; Lane4: Mouse Cerebrum lysate
Primary Ab: 4μg/ml Rabbit Anti-Human AhR Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type MCF7 cell lysate;
Lane 2: AhR knockout MCF7 cell lysate;
Predicted MW: 96kd
Observed MW: 95kd
Primary Ab: 4μg/ml Rabbit Anti-Human AhR Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: PC3 cell lysate
Primary Ab: 0.06μg/ml Rabbit Anti-Human AhR Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對AhR的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別AhR。
用法
Western blotting: 0.01-4μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB354Hu01 | 芳香烴受體(AhR)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB354Hu01 | 芳香烴受體(AhR)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB354Hu01 | 芳香烴受體(AhR)多克隆抗體 | WB; IHC; ICC/IF |
| MAB354Hu22 | 芳香烴受體(AhR)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB354Hu21 | 芳香烴受體(AhR)單克隆抗體 | IHC |
| SEB354Hu | 芳香烴受體(AhR)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB354Hu | 芳香烴受體(AhR)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB354Hu | 芳香烴受體(AhR)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Molecular and Cellular Endocrinology | Isomer-nonspecific action of dichlorodiphenyltrichloroethane on aryl hydrocarbon receptor and G-protein-coupled receptor 30 intracellular signaling in apoptotic neuronal cells[Pubmed:24859647] |
| Journal of Steroid Biochemistry and Molecular Biology | Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling[pubmed:28263910] |
| Biol Trace Elem Res | Investigation of Aryl Hydrocarbon Receptor, Zinc, and Vitamin B12 Levels in Chronic Gastritis with Helicobacter pylori Infection[33723799] |
| Int J Clin Pract | Association of aryl hydrocarbon receptor (AhR) serum level and gene rs10247158 polymorphism with anthropometric, biochemical parameters and food consumption ?-[34091989] |