腫瘤壞死因子受體超家族成員7(TNFRSF7)活性蛋白
Active Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7)
CD27; S152; T14; Tp55; CD27L receptor;
- 編號(hào)APB043Hu61
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)7.3
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
- 價(jià)格暫無報(bào)價(jià)暫無報(bào)價(jià)暫無報(bào)價(jià)暫無報(bào)價(jià)暫無報(bào)價(jià)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
TNFRSF7(Tumor necrosis factor receptor superfamily member 7), also known as CD27 antigen, is a member of the TNF-receptor superfamily. This receptor is thought to be involved in tumor necrosis factor-activated receptor activity by binding with TNFs. Thus, a binding ELISA assay was conducted to detect the association of TNFRSF7 with TNFb. Briefly, recombinant human TNFRSF7 were diluted serially in PBS with 0.01% BSA(pH 7.4). Duplicate samples of 100ul were then transferred to TNFb-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-TNFRSF7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF7 with TNFb was shown in Figure 1 and , the EC50?for this effect is 0.20ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。