轉化生長因子β1(TGFb1)活性蛋白
Active Transforming Growth Factor Beta 1 (TGFb1)
TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide
產品包裝(模擬)
產品包裝(模擬)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質量體系認證
活性實驗
Transforming growth factor beta 1 (TGF-β1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. It is reported that TGF-β1 can stimulate cell transformation by Smad signal transduction .To test the bioactivity of TGF-β1, A549 cells were seeded into 24-well plate at a density of 1x106 cells/mL, and allowed to attach overnight before treated with certain concentrations of TGF-β1. After 48 hours, the morphological changes?of?A549?were?observed by inverted microscope. The results was shown in Figure 1. A549 cells usually have a cobblestone epithelial morphology and corresponding growth pattern.? When stimulated with TGF-β1, the cells were similar with fibroblasts, the connections between cells became loose.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應用(僅供研究使用，不用于臨床診斷！) |
| APA124Bo01 | 轉化生長因子β1(TGFb1)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA124Bo01 | 轉化生長因子β1(TGFb1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA124Bo01 | 轉化生長因子β1(TGFb1)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA124Bo21 | 轉化生長因子β1(TGFb1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA124Bo | 轉化生長因子β1(TGFb1)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA124Bo | 轉化生長因子β1(TGFb1)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA124Bo | 轉化生長因子β1(TGFb1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
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| Phytomedicine | Mechanistic insights to the cardioprotective effect of Blueberry Nutraceutical Extract in Isoprenaline-Induced Cardiac Hypertrophy[] |
| Veterinary?Immunology?and?Immunopathology | Evaluation of T regulatory lymphocytes and serum concentration of selected cytokines in dogs with perianal tumors[] |
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| International Journal of?Nanomedicine | Improving the anti-keloid outcomes through liposomes loading paclitaxel–cholesterol complexes[Pubmed: 30863067] |
| Behavioural Brain Research | THE ROLE OF ANTI-INFLAMMATORY CYTOKINES IN MEMORY PROCESSING IN A HEALTHY BRAIN[] |
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| Open Access Macedonian Journal of Medical Sciences | Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells[] |
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| Food & Function | Network pharmacology oriented study reveals inflammatory state-dependent dietary supplement hepatotoxicity responses in normal and diseased rats[Pubmed: 31140472] |
| Annals of clinical and laboratory science | CD19+ CD24hiCD38hi Regulatory B cells Involved in Hepatic Alveolar Hydatid Infection in Humans[Pubmed: 31308033] |
| Life sciences | Pioglitazone attenuates kidney fibrosis via miR-21-5p modulation[Pubmed: 31254585] |
| Acta Histochemica | Hyperoside protects against heart failure-induced liver fibrosis in rats[Pubmed: 31353051] |
| Cardiology | Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart Failure[Pubmed: 31466059] |
| Scientific Reports | Anti-inflammatory and antioxidant effects of muscarinic acetylcholine receptor (mAChR) activation in the rat hippocampus[Pubmed: 31578381] |
| toxicology and applied pharmacology | miR-181b-5p inhibits endothelial-mesenchymal transition in monocrotaline-induced pulmonary arterial hypertension by targeting endocan and TGFBR1[Pubmed: 31734320] |
| Experimental Cell Research | CD4+ CD25+ Tregs as dependent factor in the course of bleomycin-induced pulmonary fibrosis in mice[Pubmed: 31678213] |
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| Biochemical and Biophysical Research Communications | Isorhynchophylline exerts anti-asthma effects in mice by inhibiting the proliferation of airway smooth muscle cells: The involvement of miR-200a-mediated FOXC1/NF?…[Pubmed: 31733831] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells[Pubmed: 32235811] |
| Journal of Oral Biology and Craniofacial Research | Levels of TGF-β1 in peri-miniscrew implant crevicular fluid[Pubmed: 32211284] |
| Acta Histochem | Paeoniflorin accelerates foot wound healing in diabetic rats though activating the Nrf2 pathway[Pubmed: 33166863] |
| EUROPEAN JOURNAL OF PHARMACOLOGY | The anti-asthmatic potential of flavonol kaempferol in an experimental model of allergic airway inflammation[Pubmed: 33122672] |
| Journal of Cosmetic Dermatology | Examination of androgenetic alopecia with serum biomarkers[Pubmed: 32969583] |
| MOLECULAR AND CELLULAR BIOCHEMISTRY | Soluble recombinant human thrombomodulin suppresses inflammation-induced gastrointestinal tumor growth in a murine peritonitis model[Pubmed: 32767229] |
| CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | Hepatoprotective effect of Linagliptin against liver fibrosis induced by carbon tetrachloride in mice[Pubmed: 32726558] |
| EXPERIMENTAL DERMATOLOGY | Decreased suppression of CD8+ and CD4+ T cells by peripheral Regulatory T cells in Generalized Vitiligo due to reduced NFATC1 and FOXP3 proteins[Pubmed: 32682346] |
| Scientific Reports | Erlotinib can halt adenine induced nephrotoxicity in mice through modulating ERK1/2, STAT3, p53 and apoptotic pathways[Pubmed: 32661331] |
| international journal of biological macromolecules | Chlorella vulgaris α-L-arabino-α-L-rhamno-α, β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effects[Pubmed: 32565301] |
| Exp Cell Res | Exosomal miR-532–5p from bone marrow mesenchymal stem cells reduce intervertebral disc degeneration by targeting RASSF5[Pubmed: 32464126] |
| JOURNAL OF IMMUNOLOGY RESEARCH | The Sufficient Immunoregulatory Effect of Autologous Bone Marrow-Derived Mesenchymal Stem Cell Transplantation on Regulatory T Cells in Patients with …[Pubmed: 32411794] |
| Frontiers in Pharmacology | M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in Mice[Pubmed: 33041798] |
| Food & Function | Nicotinamide riboside alleviates alcohol-induced depression-like behaviours in C57BL/6J mice by altering the intestinal microbiota associated with microglial?…[] |
| Effect of metformin and indole-3-carbinol on a rat model of Parkinson's disease induced by 6-hydroxydopamine[] | |
| Research square | Vitamin D Status in Dupuytren's Disease: Association with Clinical Status and Vitamin D Receptor Expression[] |
| Phytomedicine | Menthacarin attenuates experimental colitis[] |
| Immunomodulatory effects of sCD83 via induction of TGF-β1 and IDO: an in vitro and rat orthotopic liver transplantation study[] | |
| BMC Complement Med Ther | Suppression of autophagy through JAK2/STAT3 contributes to the therapeutic action of rhynchophylline on asthma[33413331] |
| Life Sci | Celecoxib ameliorates liver cirrhosis via reducing inflammation and oxidative stress along spleen-liver axis in rats[33577848] |
| CLINICAL SCIENCE | Berberine accelerated wound healing by restoring TrxR1/JNK in diabetes[33491733] |
| Bioact Mater | New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density[33842739] |
| Acta Biomater | Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia[33878473] |
| Mol Cell Biochem | Human umbilical cord-derived mesenchymal stem cells and their chondroprogenitor derivatives reduced pain and inflammation signaling and promote regeneration in??-[33864569] |
| Cell Death & Disease | Sodium/glucose cotransporter 1-dependent metabolic alterations induce tamoxifen resistance in breast cancer by promoting macrophage M2 polarization[34006822] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies[33999928] |
| Vascular pharmacology | Nectin-4 promotes lymphangiogenesis and lymphatic metastasis in breast cancer by regulating CXCR4-LYVE1 axis[33945869] |
| Equine Vet J | Limited added value of negative pressure wound therapy compared to calcium alginate dressings for second intention healing in a non?\contaminated and??-[34115409] |
| Stem Cells International | The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin??-[33381190] |
| Journal of Pharmacy and Pharmacology | Pharmacodynamic evaluation of dihydroxyflavone derivate chrysin in a guinea pig model of allergic asthma[33793800] |
| Bull. Egypt. Soc. Physiol. Sci. | Flaxseed Oil Supplementation Afford Comparable Therapeutic effect to Metformin in Bleomycin Induced Pulmonary Fibrosis Rat model[] |
| D￡?ˉ | Role of Angiotensin II and Renal Nerves on TGF-b/SmadPathway in Diabetic Nephropathy Rat Model[] |
| Bioactive Materials | Calcium phosphate-based materials regulate osteoclast-mediated osseointegration[] |
| Mediators of Inflammation | Sparstolonin B Exerts Therapeutic Effects on Collagen-Induced Arthritis by Inhibiting the NLRP3 Inflammasome and Reducing the Activity of |á1, 3 ?-[] |
| Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction[] | |
| Andrology | X‐ray micro‐computed tomography in the assessment of penile cavernous fibrosis in a rabbit castration model[34236146] |
| Aging | Yiqi Jiemin decoction alleviates allergic rhinitis in a guinea pig model by suppressing inflammation, restoring Th1/Th2 balance, and improving cellular?…[34315133] |
| Tissue Eng Regen Med | Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method[34339025] |
| Am J Transl Res | Wuling San and Xiao Chaihu Decoction affect airway inflammatory response and airway smooth muscle cell proliferation in mice with allergic asthma via miR?…[34786062] |
| Biomacromolecules | In Situ Forming Cellulose Nanofibril-Reinforced Hyaluronic Acid Hydrogel for Cartilage Regeneration[34723499] |
| Brain Sci | Neuroprotective Effect of Clobenpropit against Lipopolysaccharide-Induced Cognitive Deficits via Attenuating Neuroinflammation and Enhancing Mitochondrial?…[34942919] |
| Biomedicines | Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes[34829910] |
| J Inflamm Res | Clenbuterol, a Selective β2-Adrenergic Receptor Agonist, Inhibits or Limits Post-Stroke Pneumonia, but Increases Infarct Volume in MCAO Mice[Pubmed:35058704] |
| Cancer Commun (Lond) | Anti‐PD‐L1/TGF‐βR fusion protein (SHR‐1701) overcomes disrupted lymphocyte recovery‐induced resistance to PD‐1/PD‐L1 inhibitors in lung cancer[Pubmed:34981670] |
| Polymers (Basel) | 3D Printed Scaffold Based on Type I Collagen/PLGA_TGF-β1 Nanoparticles Mimicking the Growth Factor Footprint of Human Bone Tissue[Pubmed:35267680] |
| Biomed Pharmacother | Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis[Pubmed:35453007] |
| Biomed Pharmacother | Huangbai liniment and berberine promoted wound healing in high-fat diet/Streptozotocin-induced diabetic rats[Pubmed:35430394] |
| Antioxidants | Bio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory?…[Pubmed:35204215] |
| Frontiers in Molecular Neuroscience | Red Nucleus Interleukin-6 Evokes Tactile Allodynia in Male Rats Through Modulating Spinal Pro-inflammatory and Anti-inflammatory Cytokines[Pubmed:35465093] |
| Vaccines | Age-Dependent Dynamics of Maternally Derived Antibodies (MDAs) and Understanding MDA-Mediated Immune Tolerance in Foot-and-Mouth Disease-Vaccinated?…[Pubmed:35632433] |
| Biomaterials Advances | Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats[] |