RAS癌基因家族成員RAB7A(RAB7A)活性蛋白
Active RAB7A, Member RAS Oncogene Family (RAB7A)
RAB7; Ras-related protein Rab-7a
活性實驗

RAB7A is a ubiquitous small GTPase, which controls transport to late endocytic compartments. Silencing or overexpression of wild type RAB7A changed the soluble/insoluble rate of peripherin indicating that RAB7A is important for peripherin organization and function. Besides, disease-causing RAB7A mutant proteins bind more strongly to peripherin and their expression causes a significant increase in the amount of soluble peripherin. The altered interaction between disease-causing RAB7A mutants and peripherin could play an important role in CMT2B neuropathy. The activity of recombinant human RAB7A was measured by its ability to hydrolyze the substrate GTP to phosphate which was detected by the malachite green phosphate detection Kit (Beyotime # S0196M). The reaction was performed in the assay buffer 20 mM tris, 200 mM NaCl and 10% glycerol, pH 7.5, initiated by addition 100 μL of various concentrations of RAB7A (diluted by assay buffer) to 100 μL of 50 uM GTP. The final well serves as a negative control with no RAB7A, replaced with 100 μL assay buffer. Incubated at 37℃ for 5min, then add 70 ul phosphate detection agent and incubated at room temperature for 30min, read at a wavelength of 630 nm. The specific activity of recombinant human RAB7A is > 800 pmol/min/μg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當?shù)臈l件下存儲,損失率低于5%。
增值服務
相關產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
RPK300Hu02 | RAS癌基因家族成員RAB7A(RAB7A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APK300Hu61 | RAS癌基因家族成員RAB7A(RAB7A)活性蛋白 | Cell?culture;?Activity?Assays. |
EPK300Hu61 | RAS癌基因家族成員RAB7A(RAB7A)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APK300Hu62 | RAS癌基因家族成員RAB7A(RAB7A)活性蛋白 | Cell?culture;?Activity?Assays. |
RPK300Hu01 | RAS癌基因家族成員RAB7A(RAB7A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
SEK300Hu | RAS癌基因家族成員RAB7A(RAB7A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMK300Hu | RAS癌基因家族成員RAB7A(RAB7A)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |