蛋白S(PROS)活性蛋白
Active Protein S (PROS)
PSA; PS; PS26; PS21; PS22; PS23; PS24; PS25; VKDPS; VKDP-S; Protein Sa; Vitamin K Dependent Protein S
活性實驗

Protein S (PROS) is a vitamin K-dependent plasma protein functions as a cofactor for the anticoagulant protease, activated protein C (APC) to inhibit blood coagulation.It acts as a cofactor for activated protein C, which inactivates factors Va and VIIIa, thus reducing the risk of thrombosis.Coagulation factor VIII (F8) binds to protein S (PROS) to participate in the clotting process, regulating thrombin production and the conversion of fibrinogen to fibrin, thereby maintaining normal blood clotting function.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human PROS and recombinant dog F8.Briefly, PROS was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to F8-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PROS pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human PROS and recombinant dog F8 was shown in Figure 1, the EC50?for this effect is 0.058ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當?shù)臈l件下存儲,損失率低于5%。
增值服務
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
APB971Hu02 | 蛋白S(PROS)活性蛋白 | Cell?culture;?Activity?Assays. |
RPB971Hu01 | 蛋白S(PROS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB971Hu02 | 蛋白S(PROS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB971Hu01 | 蛋白S(PROS)多克隆抗體 | WB; IHC; ICC; IP. |
PAB971Hu02 | 蛋白S(PROS)多克隆抗體 | WB; IHC; ICC; IP. |
LAB971Hu71 | 蛋白S(PROS)多克隆抗體(生物素標記) | IHC |
MAB971Hu22 | 蛋白S(PROS)單克隆抗體 | WB; IHC; ICC; IP. |
LAB971Hu72 | 蛋白S(PROS)單克隆抗體(生物素標記) | WB; IHC; ICC. |
SEB971Hu | 蛋白S(PROS)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB971Hu | 蛋白S(PROS)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
雜志 | 參考文獻 |
Arthritis Res Ther | Validation in the ESPOIR cohort of vitamin K-dependent protein S (PROS) as a potential biomarker capable of predicting response to the methotrexate …[Pubmed:35313956] |