微纖絲關(guān)聯(lián)蛋白2(MFAP2)活性蛋白
Active Microfibrillar Associated Protein 2 (MFAP2)
MAGP1; Microfibril-associated glycoprotein 1
- 編號(hào)APC618Mu01
- 物種Mus musculus (Mouse,小鼠)相同的名稱,不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)4.8
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格10μg50μg200μg1mg5mg
- 價(jià)格¥ 2122 ¥ 5304¥ 10608¥ 31824¥ 79560
- 欲了解實(shí)際交易價(jià)格和更多情況,請(qǐng)與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
活性實(shí)驗(yàn)

Microfibrillar Associated Protein 2 (MFAP2) is an O-glycosylated protein that is secreted into the extracellular space and matrix. It plays a crucial role in connecting with biglycan and elastin in a ternary complex, supporting and maintaining the distensibility of the juxtacanalicular region of collector channels.It is reported that BGN can be combined with MFAP2 to help maintain tissue elasticity and toughness, which is particularly important for tissues such as blood vessels and skin that require a high degree of flexibility and toughness.Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse MFAP2 and recombinant rat BGN. Briefly, MFAP2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to BGN-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MFAP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse MFAP2 and recombinant rat BGN was shown in Figure 1, the EC50?for this effect is 0.031ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Mus musculus (Mouse,小鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
APC618Mu01 | 微纖絲關(guān)聯(lián)蛋白2(MFAP2)活性蛋白 | Cell?culture;?Activity?Assays. |
RPC618Mu01 | 微纖絲關(guān)聯(lián)蛋白2(MFAP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAC618Mu01 | 微纖絲關(guān)聯(lián)蛋白2(MFAP2)多克隆抗體 | WB; IHC |
SEC618Mu | 微纖絲關(guān)聯(lián)蛋白2(MFAP2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |