脂多糖結(jié)合蛋白(LBP)活性蛋白
Active Lipopolysaccharide Binding Protein (LBP)
LPS-Binding Protein
- 編號APB406Po02
- 物種Sus scrofa; Porcine (Pig，豬)相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點6.0
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
- 價格¥ 1896¥ 4740¥ 9480¥ 28440¥ 71100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
Lipopolysaccharide Binding Protein (LBP) is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). It has been reported that LBP can enhance LPS-stimulated IL-8 secretion by THP-1 cells. To test the bioactivity of recombinant pig LBP, THP-1 cells were seeded into 24-well plate at a density of 1x106 cells/mL including 1 ug/mL LPS, and treated with certain concentrations (0.625 ug/mL-5 ug/mL) of rpLBP for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of THP-1 cells increased significantly after stimulated with recombinant pig LBP have shown in Figure 1.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Sus scrofa; Porcine (Pig，豬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB406Po02 | 脂多糖結(jié)合蛋白(LBP)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB406Po03 | 脂多糖結(jié)合蛋白(LBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB406Po02 | 脂多糖結(jié)合蛋白(LBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB406Po01 | 脂多糖結(jié)合蛋白(LBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB406Po01 | 脂多糖結(jié)合蛋白(LBP)多克隆抗體 | WB; IHC; ICC; IP. |
| MAB406Po21 | 脂多糖結(jié)合蛋白(LBP)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB406Po | 脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB406Po | 脂多糖結(jié)合蛋白(LBP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| 37 | A Marker of Endotoxemia Is Associated With Obesity and Related Metabolic Disorders in Apparently Healthy Chinese[Diabetes: source] |
| AIDS | Oral serum-derived bovine immunoglobulin improves duodenal immune reconstitution and absorption function in patients with HIV enteropathy[PubMed: PMC3754419] |
| 31 | Gut microbiome composition is linked to whole grain-induced immunological improvements[Pubmed: 23038174] |
| The Veterinary Journal | High ambient temperature alleviates the inflammatory response and growth depression in pigs challenged with Escherichia coli lipopolysaccharide[Pubmed: 24792207] |
| Perfusion | The effect of normovolemic modified ultrafiltration on inflammatory mediators, endotoxins, terminal complement complexes and clinical outcome in high-risk cardiac surgery patients[Pubmed: 23429100] |
| FEMS Microbiology Ecology | A gut microbiota-targeted dietary intervention for amelioration of chronic inflammation underlying metabolic syndrome.[Pubmed: 24117923] |
| Digestive diseases and sciences | Frequency and Severity of Cirrhotic Cardiomyopathy and Its Possible Relationship with Bacterial Endotoxemia[Pubmed: 23907333] |
| Atherosclerosis | Circulating pathogen associated molecular pattern – Binding proteins and High Mobility Group Box protein 1 in nascent metabolic syndrome: Implications for cellular Toll-like receptor activity[Pubmed:25063948] |
| BMC Complementary and Alternative Medicine | Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study[Pubmed:25652121] |
| Atherosclerosis | Increased adipose tissue secretion of Fetuin-A, lipopolysaccharide-binding protein and high-mobility group box protein 1 in metabolic syndrome[PubMed: 25978344] |
| Annals of Gastroenterology | Systemic levels of human β-defensin 1 are elevated in patients with cirrhosis[PubMed: 26751578] |
| Food Chem. | Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk[Pubmed:27173528] |
| Liver International | High serum lipopolysaccharide binding protein is associated with increased mortality in patients with decompensated cirrhosis[pubmed:27712029] |
| Journal of Functional Foods | Modulation of gut microbiota and gut-generated metabolites by bitter melon results in improvement in the metabolic status in high fat diet-induced obese rats[10.1016/j.jff.2017.12.050] |
| Scientific Reports | Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains.[pubmed:28512356] |
| Journal of ethnopharmacology | A standardized extract of the fruit of Hovenia dulcis alleviated alcohol-induced hangover in healthy subjects with heterozygous ALDH2: A randomized, controlled, crossover trial.[pubmed:28750942] |
| Journal of?Allergy?and Clinical Immunology | On a Western diet, APOE ?4 is associated with low innate immune sensing, but not APOE ?3[Pubmed:29928926] |
| Journal of?Animal?Sciences | Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rations[Pubmed:30060086] |
| Journal?of?Animal?Science | Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood?…[Pubmed: 30184125] |
| BMC?Complementary and Alternative?Medicine | Evaluation of the effect of Lactobacillus reuteri V3401 on biomarkers of inflammation, cardiovascular risk and liver steatosis in obese adults with metabolic?…[Pubmed: 30453950] |
| Cell Communication and Signaling | Oridonin protects LPS-induced acute lung injury by modulating Nrf2-mediated oxidative stress and Nrf2-independent NLRP3 and NF-κB pathways[] |
| Nutrients | High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights?…[] |
| Journal of Animal Science | Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers[Pubmed: 31410465] |
| Nutrients | Lactobacillus reuteri V3401 Reduces Inflammatory Biomarkers and Modifies the Gastrointestinal Microbiome in Adults with Metabolic Syndrome: The PROSIR Study[Pubmed: 31370223] |
| journal of veterinary pharmacology and therapeutics | Effects of sinomenine in LPS‐associated diseases are related to inhibition of LBP, Mac‐1, and L‐selectin levels[Pubmed: 31490576] |
| Amyloid | Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril protein[Pubmed: 31615282] |
| Food Research International | Ethanol Extract of Propolis Prevents High-Fat Diet-Induced Insulin Resistance and Obesity in Association with Modulation of Gut Microbiota in Mice[Pubmed: 32156386] |
| Journal of Translational Medicine | Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activation[Pubmed: 33036625] |
| Communications Biology | Oxidation of fish oil exacerbates alcoholic liver disease by enhancing intestinal dysbiosis in mice[Pubmed: 32879433] |
| MOLECULAR NUTRITION & FOOD RESEARCH | Antibiotic Treatment Reduces the Health Benefits of Soy Protein[Pubmed: 32729948] |
| JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | Highly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced Obesity[Pubmed: 32633953] |
| fish & shellfish immunology | Molecular characterization and expression analysis of chemokine (CXCL12) from Nile tilapia (Oreochromis niloticus)[Pubmed: 32540504] |
| Scientific Reports | Tandem Mass Tag (TMT)-based quantitative proteomics reveals potential targets associated with onset of Sub-clinical Mastitis in cows[Pubmed: 32518370] |
| experimental eye research | Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitis[Pubmed: 32376471] |
| Frontiers in Microbiology | Naringin Attenuates High Fat Diet Induced Non-alcoholic Fatty Liver Disease and Gut Bacterial Dysbiosis in Mice[] |
| Journal of Burn Care & Research | Umbilical cord mesenchymal stem cells (hUCMSCs) for inflammatory regulation after excision and grafting of severe burn wounds in rats[33313794] |
| JOURNAL OF ANIMAL SCIENCE | Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient??-[33474564] |
| journal of agricultural and food chemistry | Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats[33497213] |
| JOURNAL OF HAZARDOUS MATERIALS | Melatonin alleviates Ochratoxin A-induced liver inflammation involved intestinal microbiota homeostasis and intestinal microbiota-independent manner[33582472] |
| Doctoral Thesis | Studies on Mastitis Caused by Translocated-Bacterial Components in Ruminants[] |
| Arthritis Res Ther | Monocyte transcriptomes from patients with axial spondyloarthritis reveal dysregulated monocytopoiesis and a distinct inflammatory imprint[34560894] |
| Microorganisms | Composition and Functions of the Gut Microbiome in Pediatric Obesity: Relationships with Markers of Insulin Resistance[34361925] |
| Antioxidants | The Prebiotic Potential of Geraniin and Geraniin-Enriched Extract against High-Fat-Diet-Induced Metabolic Syndrome in Sprague Dawley Rats[Pubmed:35453317] |
| Microbiome | Elucidating the role of the gut microbiota in the physiological effects of dietary fiber[Pubmed:35562794] |
| Bioscience of Microbiota Food and Health | Local free fatty acids trigger the expression of lipopolysaccharide-binding protein in murine white adipose tissue[Pubmed:35433160] |
| Siberian Medical Review | Сorrelation between the level of 25 (OH) D3 in blood, the content of antimicrobial peptides in oral fluid and dental caries intensity in young individuals[] |