白血病抑制因子(LIF)活性蛋白
Active Leukemia Inhibitory Factor (LIF)
CDF; D-FACTOR; HILDA; MLPLI; Cholinergic Differentiation Factor; Differentiation-Stimulating Factor; Melanoma-Derived LPL Inhibitor; Emfilermin
活性實驗

Leukemia inhibitory factor (LIF), is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. LIF as a cytokine also has another fuction including: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. The activity of LIF is usually measured by a cell proliferation assay using TF-1 cells. TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 20000 cells/well with 10% serum standard 1640?which contains various concentrations of recombinant human LIF. After incubated for 3 days,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 ℃. Proliferation of TF-1 cells after incubation with LIF for 3 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with rhLIF for 3 days. The result was shown in Figure 2. It was obvious that rhLIF significantly increased cell viability of TF-1 cells. The ED50 is 1.86 ng/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
APA085Hu61 | 白血病抑制因子(LIF)活性蛋白 | Cell?culture;?Activity?Assays. |
RPA085Hu01 | 白血病抑制因子(LIF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA085Hu61 | 白血病抑制因子(LIF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA085Hu01 | 白血病抑制因子(LIF)活性蛋白 | Cell?culture;?Activity?Assays. |
PAA085Hu01 | 白血病抑制因子(LIF)多克隆抗體 | WB; IHC |
MAA085Hu22 | 白血病抑制因子(LIF)單克隆抗體 | WB; IHC; ICC; IP. |
MAA085Hu21 | 白血病抑制因子(LIF)單克隆抗體 | IHC; ICC/IF |
MAA085Hu23 | 白血病抑制因子(LIF)單克隆抗體 | IHC |
MAA085Hu24 | 白血病抑制因子(LIF)單克隆抗體 | IHC |
SEA085Hu | 白血病抑制因子(LIF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA085Hu | 白血病抑制因子(LIF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
雜志 | 參考文獻 |
British?Journal of Anaesthesia | Leukemia inhibitory factor (LIF) potentiates antinociception activity and inhibits tolerance induction of opioids[pubmed:28077540] |
Biological Rhythm Research | A study of altered cytokine rhythms associated with successful implantation in cows[10.1080/09291016.2017.1361159] |
Integrated analysis of cancer stem cells-associated lncRNA-miRNA-mRNA network for ovarian cancer via microarray and Gene Expression Omnibus database[] |