激肽釋放酶6(KLK6)活性蛋白
Active Kallikrein 6 (KLK6)
ZYME; Bssp; Klk7; Neurosin,Zyme; PRSS18; PRSS9; SP59; HK6; Protease M; Serine protease 18
活性實驗

Tissue kallikreins are a family of extracellular serine proteases consisting of 15 members. Tissue kallikreins have attracted great interest as potential biomarkers for various cancers, including prostate, ovarian, breast, testicular, and lung. Human Kallikrein 6 (hKLK6) is a member of tissue kallikrein family observed in breast and brain tissues, colon carcinoma cells, and oligodedrocytes. Known protein substrates of hKLK6 are myelin basic protein, the precursor of the A beta amyloid peptide, and plasminogen. Its physiological functions may include the participation in demyelination processes as well as in the progression of inflammatory disease of the CNS. The activity assay of recombinant mouse KLK6 was measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC. The rmKLK6 was activated by Lysyl-endopeptidase in the activation buffer 50 mM Tris, 0.05% (w/v) Brij-35, pH 8.0, of which equal volumes of 200 ug/ml rmKLK6 and 2.5 mU/ml Lysyl-endopeptidase were combined and incubated at room temperature for 30 minutes. The activated rmKLK6 was diluted to 3 ug/ml in assay buffer and start the reaction by adding 50 μL of 200 μM substrate. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant mouse KLK6 is >6000 pmol/min/μg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當?shù)臈l件下存儲,損失率低于5%。
增值服務
相關產(chǎn)品
編號 | 適用物種:Mus musculus (Mouse,小鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
APA691Mu61 | 激肽釋放酶6(KLK6)活性蛋白 | Cell?culture;?Activity?Assays. |
EPA691Mu61 | 激肽釋放酶6(KLK6)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA691Mu02 | 激肽釋放酶6(KLK6)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA691Mu01 | 激肽釋放酶6(KLK6)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA691Mu01 | 激肽釋放酶6(KLK6)多克隆抗體 | WB; IHC; ICC; IP. |
PAA691Mu02 | 激肽釋放酶6(KLK6)多克隆抗體 | WB; IHC; ICC; IP. |
LAA691Mu81 | 激肽釋放酶6(KLK6)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
LAA691Mu71 | 激肽釋放酶6(KLK6)多克隆抗體(生物素標記) | WB; IHC; ICC. |
SEA691Mu | 激肽釋放酶6(KLK6)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA691Mu | 激肽釋放酶6(KLK6)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |