干擾素γ(IFNg)活性蛋白
Active Interferon Gamma (IFNg)
IFN-G; IFG; IFI; INFr; IFN, Immune Interferon
- 編號APA049Gu01
- 物種Cavia (Guinea pig ，豚鼠)相同的名稱，不同的物種。
- 緩沖液成份磷酸鹽緩沖液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀凍干粉
- 純度> 90%
- 等電點9.7
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Gene sequencing
SDS-PAGE圖
Figure. Western Blot; Sample: Recombinant IFNg, Cavia.
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
Interferon gamma?(IFNγ) is a?dimerized?soluble?cytokine?that is the only member of the type II class of?interferons. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. It has been reported that IFN-γ?promotes production of inducible Nitric Oxide Synthase (iNOS) in macrophages as an important activator. After stimulated with IFN-γ, morphological changes will occur in murine macrophage cell line (Raw 264.7 cells), and inducible nitric-oxide synthase (iNOS) in the cells will increase. Raw 264.7 cells were incubated in DMEM with IFN-γ (10ng/mL) for 24h, then cells?were?observed by inverted?microscope and iNOS in cell lysates was detected by ELISA.
Effect of IFN-γ on morphological change of Raw 246.7 cells was shown in Figure 1.
Effect of IFN-γ on the expression of iNOS was shown in Table 1.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Cavia (Guinea pig ，豚鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA049Gu01 | 干擾素γ(IFNg)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA049Gu01 | 干擾素γ(IFNg)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA049Gu01 | 干擾素γ(IFNg)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA049Gu91 | 干擾素γ(IFNg)多克隆抗體(辣根過氧化物酶標(biāo)記) | WB; IHC; ICC. |
| LAA049Gu71 | 干擾素γ(IFNg)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA049Gu21 | 干擾素γ(IFNg)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA049Gu | 干擾素γ(IFNg)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA049Gu | 干擾素γ(IFNg)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA049Gu | 干擾素γ(IFNg)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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| Melatonin-mediated MT2 attenuates colitis induced by dextran sodium sulfate via PI3K/AKT/Nrf2/SIRT1/ROR|á/NF-|êB signaling pathways[34162146] | |
| How Does the Th1/Th2 Polarization in Milk Change According to the Types of Bacteria that Cause Subclinical Mastitis in Cows?[] | |
| Amplified Antitumor Efficacy By A Targeted Drugs Retention and Chemosensitization Strategies-Based ?°Combo?± Nanoagent Together With PD-L1 Blockade In??-[] | |
| World J Tradit Chin Med | Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction via Th2 cytokine enhancement[] |
| Cancer Lett | Photodynamic therapy induces human esophageal carcinoma cell pyroptosis by targeting the PKM2/caspase-8/caspase-3/GSDME axis[34256094] |
| J Pharm Pharmacol | Alamandine protects against renal ischaemia–reperfusion injury in rats via inhibiting oxidative stress[34244746] |
| J Nanobiotechnology | Amplified antitumor efficacy by a targeted drug retention and chemosensitization strategy-based “combo” nanoagent together with PD-L1 blockade in?…[34225744] |
| Neoplasia | PDIA6 promotes pancreatic cancer progression and immune escape through CSN5-mediated deubiquitination of β-catenin and PD-L1[34325342] |
| Sci Rep | Talaromyces marneffei and nontuberculous mycobacteria co-infection in HIV-negative patients[34376749] |
| Am J Transl Res | Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma[34377237] |
| J Immunol Res | Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus[34395633] |
| Sci Rep | Novel interferon-sensitive genes unveiled by correlation-driven gene selection and systems biology[34508139] |
| Transl Psychiatry | Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive?…[34802039] |
| Infect Drug Resist | Retrospective Analysis of 10 Cases of Disseminated Nontuberculous Mycobacterial Disease with Osteolytic Lesions[34785914] |
| Environmental Research | In-vitro oxidative potential and inflammatory response of ambient PM2. 5 in a rural region of Northwest China: Association with chemical compositions and?…[34863982] |
| Eur J Pharm Sci | Preclinical study of methotrexate-based hydrogels versus surfactant based liquid crystal systems on psoriasis treatment[34314841] |
| J Inflamm Res | Clenbuterol, a Selective β2-Adrenergic Receptor Agonist, Inhibits or Limits Post-Stroke Pneumonia, but Increases Infarct Volume in MCAO Mice[Pubmed:35058704] |
| Poult Sci | Salmonella phage CKT1 significantly relieves the body weight loss of chicks by normalizing the abnormal intestinal microbiome caused by hypervirulent?…[Pubmed:35063807] |
| ACS Appl Mater Interfaces | Combinational Chemoimmunotherapy for Breast Cancer by Codelivery of Doxorubicin and PD-L1 siRNA Using a PAMAM-Incorporated Liposomal Nanoplatform[Pubmed:35138103] |
| Theranostics | Combating multidrug resistance and metastasis of breast cancer by endoplasmic reticulum stress and cell-nucleus penetration enhanced?…[Pubmed:35401832] |
| Inflammopharmacology | Silymarin constrains diacetyl-prompted oxidative stress and neuroinflammation in rats: involvements of Dyn/GDNF and MAPK signaling pathway[Pubmed:35366745] |
| Journal of Nanobiotechnology | Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM[Pubmed:35351131] |
| Infection and Drug Resistance | Sweet's Syndrome Accompanied by Coinfection with Multiple Pathogens and Disseminated Mycobacterium phlei Infection Presenting with Osteolytic?…[Pubmed:35592103] |
| Sci Rep | Clinical findings and predictive factors for positive anti-interferon-γ autoantibodies in patients suffering from a non-tuberculosis mycobacteria or Talaromyces?…[Pubmed:35641599] |
| Biomedicines | Deletion of Toxoplasma Rhoptry Protein 38 (PruΔrop38) as a Vaccine Candidate for Toxoplasmosis in a Murine Model[Pubmed:35740356] |
| Viruses | Synergistic Pathogenicity by Coinfection and Sequential Infection with NADC30-like PRRSV and PCV2 in Post-Weaned Pigs[Pubmed:35215787] |
| Antioxidants | Blue Light Alters the Composition of the Jejunal Microbiota and Promotes the Development of the Small Intestine by Reducing Oxidative Stress[Pubmed:35204158] |
| Oxidative Medicine and Cellular Longevity | The Role of Aeromonas-Goblet Cell Interactions in Melatonin-Mediated Improvements in Sleep Deprivation-Induced Colitis[Pubmed:35355860] |
| Journal of Integrative Medicine | Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats[Pubmed:35643766] |
| researchsquare | MSC-EVs: A Promising Modulator for Immune Response in Systemic Lupus Erythematosus[] |
| Mljekarstvo | Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows[] |