半乳糖凝集素1(GAL1)活性蛋白
Active Galectin 1 (GAL1)
LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin
- 編號APA321Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)5.1
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
Galectins are a family of carbohydrate-binding proteins with an affinity for beta-galactosides. Galectin-1 (GAL1) is differentially expressed by various normal and pathological tissues and appears to be functionally polyvalent, with a wide range of biological activity. The intracellular and extracellular activity of GAL1 has been described. Evidence points to GAL1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.It also can agglutinate red blood. In this case, chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant human GAL1 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL1 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL1, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL1 is 2.5μg/mL.
Figure 2. The hemagglutination assay of GAL1 in V- bottom shaped 96-well microtiter plate.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA321Hu01 | 半乳糖凝集素1(GAL1)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA321Hu01 | 半乳糖凝集素1(GAL1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA321Hu01 | 半乳糖凝集素1(GAL1)多克隆抗體 | WB; IHC; ICC/IF; FCM |
| FAA321Hu01 | 抗半乳糖凝集素1(GAL1)多克隆抗體 | FCM |
| LAA321Hu71 | 半乳糖凝集素1(GAL1)多克隆抗體(生物素標(biāo)記) | WB |
| LAA321Hu81 | 半乳糖凝集素1(GAL1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA321Hu22 | 半乳糖凝集素1(GAL1)單克隆抗體 | IHC |
| MAA321Hu21 | 半乳糖凝集素1(GAL1)單克隆抗體 | WB |
| MEA321Hu | 半乳糖凝集素1(GAL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA321Hu | 半乳糖凝集素1(GAL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA321Hu | 半乳糖凝集素1(GAL1)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA321Hu | 半乳糖凝集素1(GAL1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA321Hu01 | 半乳糖凝集素1(GAL1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| International Journal of Oncology | Galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: Regulatory roles of cell surface glycans[Pubmed:24589677] |
| Clinica Chimica Acta | Galectin-1 and galectin-3: plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population[Pubmed:25578395] |
| Journal of Perinatal Medicine | Cord blood galectin-1 and-3 concentrations in term pregnancies with normal restricted and increased fetal growth[PubMed: 24717333] |
| Biomaterials | Noninvasive small-animal imaging of galectin-1 upregulation for predicting tumor resistance to radiotherapy[Pubmed:29272764] |
| Advances in Experimental?Medicine?and?Biology | Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease[Pubmed:29429028] |
| RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY | Elevated level of Galectin-1 in bronchoalveolar lavage of patients with idiopathic pulmonary fibrosis[Pubmed: 31678459] |
| Eur J Nucl Med Mol Imaging | Galectin expression detected by 68Ga-galectracer PET as a predictive biomarker of radiotherapy resistance[Pubmed:35106644] |
| Medicine | Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive?…[Pubmed:35687771] |
| In Vitro Bovine Embryo Production Using Medium's Supplemented with Recombinant Human Galectin-1 Buffered[] |