甘丙肽(GAL)活性蛋白
Active Galanin (GAL)
GALN; GLNN; GMAP; Galanin Prepropeptide; Galanin-Message-Associated Peptide
產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質量體系認證
活性實驗
Galanin (GAL) is a neuropeptide widely distributed in the central and peripheral nervous systems. It consists of 29 or 30 amino acids and is involved in various physiological processes like regulating appetite, pain sensation, memory, and endocrine function.Besides,Galanin Like Peptide (GALP) has been identified as an interactor of GAL, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GAL and recombinant rat GALP. Briefly, biotin-linked GAL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to GALP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μl stop solution to the wells and read at 450nm immediately. The binding activity of GAL and GALP was shown in Figure 1, the EC50?for this effect is 0.282 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APB084Hu02 | 甘丙肽(GAL)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB084Hu02 | 甘丙肽(GAL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB084Hu01 | 甘丙肽(GAL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPB084Hu21 | 甘丙肽(GAL)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAB084Hu01 | 甘丙肽(GAL)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB084Hu81 | 甘丙肽(GAL)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAB084Hu71 | 甘丙肽(GAL)多克隆抗體(生物素標記) | WB |
| MAB084Hu22 | 甘丙肽(GAL)單克隆抗體 | WB; IHC; ICC; IP. |
| CEB084Hu | 甘丙肽(GAL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCB084Hu | 甘丙肽(GAL)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB084Hu | 甘丙肽(GAL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB084Hu11 | 甘丙肽(GAL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Progress in Neuro-Psychopharmacology and Biological Psychiatry | Decreased galanin serum levels are associated with alcohol-craving during withdrawal[ScienceDirect: S0278584610005191] |
| Clinical Biochemistry | Circulating galanin levels are increased in patients with gestational diabetes mellitus[PubMed: 23266294] |
| Peptides | Endogenous galanin as a novel biomarker to predict gestational diabetes mellitus.[Pubmed: 24503374] |
| Gynecological Endocrinology | Circulating galanin and IL-6 concentrations in gestational diabetes mellitus[Pubmed:24397394] |
| Eat Behav | Association between circulating levels of galanin and pre-pregnancy body mass index in patients with gestational diabetes mellitus[PubMed: 26172564] |
| Cell Biochemistry and Biophysics | The Effects of Levetiracetam on Cerebrospinal Fluid and Plasma NPY and GAL, and on the Components of Stress Response System, hs-CRP, and S100B Protein in Serum of Patients with Refractory Epilepsy[Article: 10.1007] |
| Journal of Addiction & Prevention | Low Galanin Serum Levels are Associated with Anxiety in Opioid-Maintained Patients[688687ca8d5a1d03ed060f761328001ca673.pdf] |
| Oncology Letter | Colorectal cancer patients exhibit increased levels of galanin in serum and colon tissues[pubmed:27899999] |
| Scientific Reports | Serum?Galanin?Levels?in?Young?Healthy?Lean?and?Obese?Non-Diabetic?Men?during an?OralGlucose?Tolerance?Test.[pubmed:27550417] |
| Journal of?Clinical?Investigation? | Adrenomedullin and Galanin Responses to Orthostasis in Older Persons [pubmed:28796366] |
| Child Psychiatry Hum Dev | Serum Galanin in Children with Autism Spectrum Disorder[33534034] |