粒細胞巨噬細胞集落刺激因子(GM-CSF)活性蛋白
Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)
CSF2; GMCSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor
- 編號APA045Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點5.9
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
SDS-PAGE圖
Figure . Western Blot; Sample: APA045Mu01; Antibody:PAA045Mu01
通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Measured in a cell proliferation assay using mouse BMDC (bone marrow derived dendritic cells). The ED50 (median effective dose) for this effect is less than 0.25 ng/mL.
In-house data of APA045Mu01 used in cellular experiment:
Six-eight weeks old Balb/c mice were used for BMDC. At first, mouse femur and tibia were taken out, and then bone marrow was washed out with serum-free RPMI 1640 medium, followed by centrifugation at 1200RPM for 5min (4oC). ACK buffer was added to get rid of red blood cells, and then, centrifuged at 1200RPM for 5min (4oC). Cell pellets were collected and re-suspended, the cells were cultured in DMEM medium supplemented with 10% FBS, or DMEM medium supplemented with 10% FBS and GMCSF (APA045Mu01) at 37oC with 5% CO2 in thermostatic incubator. Three days later, cells were observed by microscope, and the result is shown in Figure 1.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| EPA045Mu61 | 粒細胞巨噬細胞集落刺激因子(GM-CSF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA045Mu02 | 粒細胞巨噬細胞集落刺激因子(GM-CSF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA045Mu01 | 粒細胞巨噬細胞集落刺激因子(GM-CSF)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA045Mu61 | 粒細胞巨噬細胞集落刺激因子(GM-CSF)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA045Mu01 | 粒細胞巨噬細胞集落刺激因子(GM-CSF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA045Mu01 | 粒細胞巨噬細胞集落刺激因子(GM-CSF)多克隆抗體 | WB; IHC |
| PAA045Mu06 | 粒細胞巨噬細胞集落刺激因子(GM-CSF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA045Mu02 | 粒細胞巨噬細胞集落刺激因子(GM-CSF)多克隆抗體 | WB; IHC; ICC; IP. |
| SEA045Mu | 粒細胞巨噬細胞集落刺激因子(GM-CSF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA045Mu | 粒細胞巨噬細胞集落刺激因子(GM-CSF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA045Mu | 粒細胞巨噬細胞集落刺激因子(GM-CSF)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA045Mu | 粒細胞巨噬細胞集落刺激因子(GM-CSF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA045Mu01 | 粒細胞巨噬細胞集落刺激因子(GM-CSF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Toxicology and Environmental Health | Effects of 900-MHz Microwave Radiation on γ-Ray-Induced Damage to Mouse Hematopoietic System[PubMed: 20391130] |
| 5 | Safety and efficacy of the peptide-based therapeutic vaccine for HIV-1, Vacc-4×: a phase 2 randomised, double-blind, placebo-controlled trial[Pubmed:24525316] |
| Archives of Virology | Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central China[Pubmed:29305646] |
| BioMed Research International | Activated Macrophages of Monocytic Origin Predominantly Express Proinflammatory Cytokine Genes, Whereas Kupffer Cells Predominantly Express Anti?…[] |
| bulletin of experimental biology and medicine | Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin[Pubmed: 31183654] |
| Exp Mol Pathol | The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal??-[33285209] |
| Vaccine | Co-delivery of PSMA antigen epitope and mGM-CSF with a cholera toxin-like chimeric protein suppressed prostate tumor growth via activating dendritic cells and??-[33612342] |