過氧化氫酶(CAT)活性蛋白
Active Catalase (CAT)
- 編號(hào)APC418Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)7.5
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
- 價(jià)格¥ 1502¥ 3756¥ 7512¥ 22536¥ 56340
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Catalase (CAT) is an antioxidant enzyme present in all aerobic organisms. It is known to catalyze H2O2 into water and oxygen in an energy-efficient manner in the cells exposed to environmental stress. H2O2 will have specific absorbance at 240 nm . when we add CAT， the absorbance wil decrease, thus the activiy of CAT can be measured by caculating H2O2 absorbance decrease. The reaction was performed in adding 10ul (dilute with 50mM Potassium Phosphate Buffer, pH 7.0) recombinant Rat CAT to 290ul substrate mixture solution (50mM Potassium Phosphate Buffer, pH 7.0, 0.036% H2O2, allow the substrate to equilibrate to 25 °C), quickly mixed, then record the time required for the A240 to decrease from 0.45 to 0.40 absorbance units. One unit of catalase will decompose 1.0 μmole of H2O2 per minute at pH 7.0 at 25 °C. The activity of recombiant Rat CAT is 176000 U/mg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| NPC418Ra01 | 過氧化氫酶(CAT)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC418Ra01 | 過氧化氫酶(CAT)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPC418Ra01 | 過氧化氫酶(CAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC418Ra01 | 過氧化氫酶(CAT)多克隆抗體 | WB |
| PAC418Ra05 | 過氧化氫酶(CAT)多克隆抗體 | WB |
| MAC418Ra21 | 過氧化氫酶(CAT)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC418Ra | 過氧化氫酶(CAT)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC418Ra | 過氧化氫酶(CAT)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
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